Human MMP-9(Matrix Metalloproteinase 9) ELISA Kit
中文名称:人基质金属蛋白酶9(MMP-9)酶联免疫吸附测定试剂盒
别称:
MMP9、CLG4B、Gelatinase B、GELB、MANDP2、92kDa Type IV Collagenase、92 KDa Gelatinase
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 0.16-10 ng/mL
- 灵敏度: 0.1 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人MMP-9抗体包被于酶标板上,实验时样品(或标准品)中的人MMP-9会与包被抗体结合。后依次加入生物素化的抗人MMP-9抗体和辣根过氧化物酶标记的亲和素,抗人MMP-9抗体与结合在包被抗体上的人MMP-9结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,MMP-9浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中MMP-9的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | Colormetric |
检测范围 | 0.16-10 ng/mL |
灵敏度 | 0.1 ng/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的人MMP-9,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Salivary MMP-9 Levels in Chronic Periodontitis Patients with Type-II Diabetes Mellitus
IF: 4.412 -
Anti-proliferation and anti-inflammation effects of corilagin in rheumatoid arthritis by downregulating NF-κB and MAPK signaling pathways
IF: 4.36 -
Effect of levetiracetam in combination with topiramate on immune function, cognitive function, and neuronal nutritional status of children with intractable epilepsy
IF: 4.06Journal:American Journal of Translational ResearchPMID:34650715 -
Association between isotretinoin (Roaccutanne) use and changes in periodontal clinical parameters and MMP-8 and MMP-9 salivary levels.
IF: 4.009Journal:Frontiers in Bioscience-LandmarkDOI:10.52586/4933 -
Identification of NCK1 as a novel downstream effector of STAT3 in colorectal cancer metastasis and angiogenesis
IF: 3.937Journal:CELLULAR SIGNALLING -
Continuous activation of Nrf2 and its target antioxidant enzymes leads to arsenite-induced malignant transformation of human bronchial epithelial cells
IF: 3.705Journal:TOXICOLOGY AND APPLIED PHARMACOLOGY -
Trehalose against UVB-induced skin photoaging by suppressing MMP expression and enhancing procollagen I synthesis in HaCaT cells
IF: 3.701Journal:Journal of Functional Foods -
Lutein Treatment Effects on the Redox Status and Metalloproteinase-9 (MMP-9) in Oral Cancer Squamous Cells—Are There Therapeutical Hopes?
IF: 3.623Journal:Materials -
Fenofibrate mediated activation of PPARα negatively regulates trophoblast invasion
IF: 3.481 -
Obesity, Metabolic syndrome, Diabetes
IF: 3.432Journal:CLINICAL CHEMISTRY AND LABORATORY MEDICINE