Mouse IL-10(Interleukin 10) ELISA Kit
中文名称:小鼠白介素10(IL-10)酶联免疫吸附测定试剂盒
别称:
IL10、IL10A、CSIF、TGIF
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 15.63-1000 pg/mL
- 灵敏度: 9.38 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-10抗体包被于酶标板上,实验时样品(或标准品)中的小鼠IL-10会与包被抗体结合。后依次加入生物素化的抗小鼠IL-10抗体和辣根过氧化物酶标记的亲和素,抗小鼠IL-10抗体与结合在包被抗体上的小鼠IL-10结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IL-10浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-10的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | Colormetric |
检测范围 | 15.63-1000 pg/mL |
灵敏度 | 9.38 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的小鼠IL-10,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Flaxseed oligosaccharides alleviate DSS-induced colitis through modulation of gut microbiota and repair of the intestinal barrier in mice
IF: 4.171 -
Preventive and synbiotic effects of the soluble dietary fiber obtained from Lentinula edodes byproducts and Lactobacillus plantarum LP90 against dextran sulfate sodium-induced colitis in mice
IF: 4.125 -
Cannabinoid Analogue WIN 55212–2 Protects Paraquat-Induced Lung Injury and Enhances Macrophage M2 Polarization
IF: 4.092Journal:INFLAMMATION -
Ex vivo tolerization and M2 polarization of macrophages dampens both pro- and anti-inflammatory cytokine production in response to diabetic wound fluid stimulation
IF: 4.079Journal:BIOCHIMIE -
Oral Immunization with Recombinant Lactobacillus acidophilus Expressing espA-Tir-M Confers Protection against Enterohemorrhagic Escherichia coli O157:H7 Challenge in Mice
IF: 4.076Journal:Frontiers in Microbiology -
Myricitrin pretreatment ameliorates mouse liver ischemia reperfusion injury
IF: 3.943 -
Molecular hydrogen attenuates sepsis-induced neuroinflammation through regulation of microglia polarization through an mTOR-autophagy-dependent pathway
IF: 3.943 -
Didymin ameliorates dexamethasone-induced non-alcoholic fatty liver disease by inhibiting TLR4/NF-κB and PI3K/Akt pathways in C57BL/6J mice
IF: 3.943 -
Cardiomyocyte-specific deletion of Senp2 contributes to CVB3 viral replication and inflammation
IF: 3.943 -
Transcriptome profiling of tolerogenic dendritic cells conditioned with dual mTOR kinase inhibitor, AZD8055
IF: 3.943Journal:INTERNATIONAL IMMUNOPHARMACOLOGY