Mouse IL-6(Interleukin 6) ELISA Kit
中文名称:小鼠白介素6(IL-6)酶联免疫吸附测定试剂盒
别称:
IL6、BSF2、HGF、HSF、IFNB2、CDF
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 31.25-2000 pg/mL
- 灵敏度: 18.75 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-6抗体包被于酶标板上,实验时样品(或标准品)中的小鼠IL-6会与包被抗体结合。后依次加入生物素化的抗小鼠IL-6抗体和辣根过氧化物酶标记的亲和素,抗小鼠IL-6抗体与结合在包被抗体上的小鼠IL-6结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IL-6浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-6的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | Colormetric |
检测范围 | 31.25-2000 pg/mL |
灵敏度 | 18.75 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的小鼠IL-6,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Roles of Crosstalk between Astrocytes and Microglia in Triggering Neuroinflammation and Brain Edema Formation in 1,2-Dichloroethane-Intoxicated Mice
IF: 6.6 -
Anti-Neuroinflammatory Effect of Alantolactone through the Suppression of the NF-κB and MAPK Signaling Pathways
IF: 6.6Journal:Cells -
The Protective Effect of Sulforaphane on Dextran Sulfate Sodium-Induced Colitis Depends on Gut Microbial and Nrf2-Related Mechanism
IF: 6.59Journal:Frontiers in Nutrition -
Using Collagen Peptides From the Skin of Monkfish (Lophius litulon) to Ameliorate Kidney Damage in High-Fat Diet Fed Mice by Regulating the Nrf2 Pathway and NLRP3 Signaling
IF: 6.576Journal:Frontiers in Nutrition -
Exposure to hyperandrogen drives ovarian dysfunction and fibrosis by activating the NLRP3 inflammasome in mice
IF: 6.551Journal:SCIENCE OF THE TOTAL ENVIRONMENT -
Preventive Effect of Collagen Peptides from Acaudina molpadioides on Acute Kidney Injury through Attenuation of Oxidative Stress and Inflammation
IF: 6.543 -
Low-Dose Copper Exposure Exacerbates Depression-Like Behavior in ApoE4 Transgenic Mice
IF: 6.543 -
Hydrogen Sulfide Mitigates Kidney Injury in High Fat Diet-Induced Obese Mice
IF: 6.543Journal:Oxidative Medicine and Cellular Longevity -
Activation of α7nAChR preserves intestinal barrier integrity by enhancing the HO-1 / STAT3 signaling to inhibit NF-κB activation in mice
IF: 6.53 -
Inhibition of HDAC6 attenuates LPS-induced inflammation in macrophages by regulating oxidative stress and suppressing the TLR4-MAPK/NF-κB pathways
IF: 6.53