Mouse VEGF-A(Vascular Endothelial Cell Growth Factor A) ELISA Kit
中文名称:小鼠血管内皮细胞生长因子A(VEGF-A)酶联免疫吸附测定试剂盒
别称:
VEGF-A、VEGFA、MVCD1、VPF
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 31.25-2000 pg/mL
- 灵敏度: 18.75 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠VEGF-A抗体包被于酶标板上,实验时样品(或标准品)中的小鼠VEGF-A会与包被抗体结合。后依次加入生物素化的抗小鼠VEGF-A抗体和辣根过氧化物酶标记的亲和素,抗小鼠VEGF-A抗体与结合在包被抗体上的小鼠VEGF-A结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,VEGF-A浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中VEGF-A的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | Colormetric |
检测范围 | 31.25-2000 pg/mL |
灵敏度 | 18.75 pg/mL |
样本体积 | 100μL |
样本类型 | 血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的小鼠VEGF-A,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Oroxylin a Attenuates Limb Ischemia by Promoting Angiogenesis via Modulation of Endothelial Cell Migration
IF: 5.811 -
Targeted delivery of zoledronic acid through the sialic acid - Siglec axis for killing and reversal of M2 phenotypic tumor-associated macrophages – A promising cancer immunotherapy
IF: 4.845Journal:INTERNATIONAL JOURNAL OF PHARMACEUTICS -
Shuxuetong injection and its peptides enhance angiogenesis after hindlimb ischemia by activating the MYPT1/LIMK1/Cofilin pathway
IF: 4.36 -
The Chinese medicine Fufang Zhenzhu Tiaozhi capsule protects against atherosclerosis by suppressing EndMT via modulating Akt1/β-catenin signaling pathway
IF: 4.36 -
Immunological consequences of compromised ocular immune privilege accelerate retinal degeneration in retinitis pigmentosa
IF: 4.303 -
Intraperitoneal injection of thalidomide alleviates early osteoarthritis development by suppressing vascular endothelial growth factor expression in mice
IF: 1.922Journal:Molecular Medicine Reports -
VEGF secreted by mesenchymal stem cells mediates the differentiation of endothelial progenitor cells into endothelial cells via paracrine mechanisms
IF: 1.922Journal:Molecular Medicine Reports