MS Mouse IFN-γ(Interferon Gamma) ELISA Kit
中文名称:MS-小鼠γ干扰素(IFN-γ)酶联免疫吸附测定试剂盒
别称:
IFN-γ
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 7.81-500 pg/mL
- 灵敏度: 4.53 pg/mL
系列介绍 | MS为Mini Sample的缩写。MS系列试剂盒的上样量仅为传统夹心法ELISA试剂盒的1/4,能有效解决实验样本体积不够的问题。Elabscience®自主开发的新技术,旨在帮助您以更高效的方式进行科学研究。 |
用途 | 试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中IFN-γ浓度 |
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IFN-γ抗体包被于酶标板上,实验时样品(或标准品)中的小鼠IFN-γ会与包被抗体结合。后依次加入生物素化的抗小鼠IFN-γ抗体和辣根过氧化物酶标记的亲和素,抗小鼠IFN-γ抗体与结合在包被抗体上的小鼠IFN-γ结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IFN-γ浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IFN-γ的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | Colormetric |
检测范围 | 7.81-500 pg/mL |
灵敏度 | 4.53 pg/mL |
样本体积 | 25μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的小鼠IFN-γ,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Self-Activated Cascade Biocatalysis of Glucose Oxidase–Polycation–Iron Nanoconjugates Augments Cancer Immunotherapy
IF: 10.383Journal:ACS Applied Materials & Interfaces -
Inflammation-Regulated Nanodrug Sensitizes Hepatocellular Carcinoma to Checkpoint Blockade Therapy by Reprogramming the Tumor Microenvironment
IF: 10.383 -
MIF-inhibition alleviates vitiligo progression by suppressing CD8+T cell activation and proliferation
IF: 9.883Journal:JOURNAL OF PATHOLOGY -
Microsomal prostaglandin E synthase-1 inhibition prevents adverse cardiac remodeling after myocardial infarction in mice
IF: 9.473Journal:BRITISH JOURNAL OF PHARMACOLOGY -
Inorganic Nanozyme with Combined Self-Oxygenation/Degradable Capabilities for Sensitized Cancer Immunochemotherapy
IF: 9.043Journal:Nano-Micro Letters -
Oral administration of Bacillus cereus GW-01 alleviates the accumulation and detrimental effects of β-cypermethrin in mice
IF: 8.943Journal:CHEMOSPHERE -
Gut Microflora Modulates Th17/Treg Cell Differentiation in Experimental Autoimmune Prostatitis via the Short-Chain Fatty Acid Propionate
IF: 8.786 -
Engineering blood exosomes for tumor-targeting efficient gene/chemo combination therapy
IF: 8.579 -
Successful Treatment of Vitiligo with Cold Atmospheric Plasma‒Activated Hydrogel
IF: 8.551 -
Carbon ion radiotherapy triggers immunogenic cell death and sensitizes melanoma to anti-PD-1 therapy in mice
IF: 8.11
E-EL-M0048 | Mouse IFN-γ(Interferon Gamma) ELISA Kit | 96T , 48T , 96T*5 |