MS Mouse IL-10(Interleukin 10) ELISA Kit
中文名称:MS-小鼠白介素10(IL-10)酶联免疫吸附测定试剂盒
别称:
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 15.63-1000 pg/mL
- 灵敏度: 9.13 pg/mL
系列介绍 | MS为Mini Sample的缩写。MS系列试剂盒的上样量仅为传统夹心法ELISA试剂盒的1/4,能有效解决实验样本体积不够的问题。Elabscience®自主开发的新技术,旨在帮助您以更高效的方式进行科学研究。 |
用途 | 试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中IL-10浓度 |
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-10抗体包被于酶标板上,实验时样品(或标准品)中的小鼠IL-10会与包被抗体结合。后依次加入生物素化的抗小鼠IL-10抗体和辣根过氧化物酶标记的亲和素,抗小鼠IL-10抗体与结合在包被抗体上的小鼠IL-10结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IL-10浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-10的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 15.63-1000 pg/mL |
灵敏度 | 9.13 pg/mL |
样本体积 | 25μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的小鼠IL-10,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
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Activation of α7nAChR preserves intestinal barrier integrity by enhancing the HO-1 / STAT3 signaling to inhibit NF-κB activation in mice
IF: 6.53 -
Silica particles disorganize the polarization of pulmonary macrophages in mice
IF: 6.291 -
Diet composition affects long-term zearalenone exposure on the gut–blood–liver axis metabolic dysfunction in mice
IF: 6.291Journal:ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY -
Rice Protein Peptides Alleviate Dextran Sulfate Sodium-Induced Colitis via the Keap1–Nrf2 Signaling Pathway and Regulating Gut Microbiota
IF: 5.895Journal:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY -
Erianin inhibits human lung cancer cell growth via PI3K/Akt/mTOR pathway in vitro and in vivo
IF: 5.882 -
Sequential administration of sialic acid-modified liposomes as carriers for epirubicin and zoledronate elicit stronger antitumor effects with reduced toxicity
IF: 5.875 -
The Effect of Shionone on Sepsis-Induced Acute Lung Injury by the ECM1/STAT5/NF-κB Pathway
IF: 5.811Journal:Frontiers in Pharmacology -
JMJD3/H3K27me3 epigenetic modification regulates Th17/Treg cell differentiation in ulcerative colitis
IF: 5.714 -
Targeting PERK mediated endoplasmic reticulum stress attenuates neuroinflammation and alleviates lipopolysaccharide-induced depressive-like behavior in male mice
IF: 5.714 -
CHIP ameliorates cerebral ischemia-reperfusion injury by attenuating necroptosis and inflammation
IF: 5.682
E-EL-M0046 | Mouse IL-10(Interleukin 10) ELISA Kit | 96T , 48T , 96T*5 |