MS Mouse IL-10(Interleukin 10) ELISA Kit
中文名称:MS-小鼠白介素10(IL-10)酶联免疫吸附测定试剂盒
别称:
IL10、IL10A、CSIF、TGIF
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 15.63-1000 pg/mL
- 灵敏度: 9.13 pg/mL
系列介绍 | MS为Mini Sample的缩写。MS系列试剂盒的上样量仅为传统夹心法ELISA试剂盒的1/4,能有效解决实验样本体积不够的问题。Elabscience®自主开发的新技术,旨在帮助您以更高效的方式进行科学研究。 |
用途 | 试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中IL-10浓度 |
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-10抗体包被于酶标板上,实验时样品(或标准品)中的小鼠IL-10会与包被抗体结合。后依次加入生物素化的抗小鼠IL-10抗体和辣根过氧化物酶标记的亲和素,抗小鼠IL-10抗体与结合在包被抗体上的小鼠IL-10结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IL-10浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-10的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | Colormetric |
检测范围 | 15.63-1000 pg/mL |
灵敏度 | 9.13 pg/mL |
样本体积 | 25μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的小鼠IL-10,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Conditioned medium of mesenchymal stem cells pretreated with H2O2 promotes intestinal mucosal repair in acute experimental colitis
IF: 4.996Journal:Scientific Reports -
CaMK4‐dependent phosphorylation of Akt/mTOR underlies Th17 excessive activation in experimental autoimmune prostatitis
IF: 4.966Journal:FASEB JOURNAL -
MiR-146a modulates macrophage polarization by inhibiting Notch1 pathway in RAW264.7 macrophages
IF: 4.932Journal:INTERNATIONAL IMMUNOPHARMACOLOGY -
Oxymatrine inhibits neuroinflammation by Regulating M1/M2 polarization in N9 microglia through the TLR4/NF-κB pathway
IF: 4.932Journal:INTERNATIONAL IMMUNOPHARMACOLOGY -
IL-25 improves diabetic wound healing through stimulating M2 macrophage polarization and fibroblast activation
IF: 4.932 -
Atractyloside-A ameliorates spleen deficiency diarrhea by interfering with TLR4/MyD88/NF-κB signaling activation and regulating intestinal flora homeostasis
IF: 4.932 -
Arsenic trioxide improves Treg and Th17 balance by modulating STAT3 in treatment-naïve rheumatoid arthritis patients
IF: 4.932Journal:INTERNATIONAL IMMUNOPHARMACOLOGY -
Fine particulate matter increases airway hyperresponsiveness through kallikrein-bradykinin pathway
IF: 4.872 -
Targeting IL-17A Improves the Dysmotility of the Small Intestine and Alleviates the Injury of the Interstitial Cells of Cajal during Sepsis
IF: 4.868 -
Therapeutic potential of nanolipoidal α-terpineol in combating keratitis induced by Pseudomonas aeruginosa in the murine model
IF: 4.845
E-EL-M0046 | Mouse IL-10(Interleukin 10) ELISA Kit | 96T , 48T , 96T*5 |