MS Mouse IL-17A(Interleukin 17A) ELISA Kit
中文名称:MS-小鼠白介素17A(IL-17A)酶联免疫吸附测定试剂盒
别称:
CTLA8、CTLA-8、CTLA8cytotoxic T-lymphocyte-associated serine esterase 8、Cytotoxic T-lymphocyte-associated antigen 8、IL17、IL-17、IL17A、IL-17A、IL-17Acytotoxic T-lymphocyte-associated protein 8、IL-17CTLA-8
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 7.81-500 pg/mL
- 灵敏度: 2.19 pg/mL
系列介绍 | MS为Mini Sample的缩写。MS系列试剂盒的上样量仅为传统夹心法ELISA试剂盒的1/4,能有效解决实验样本体积不够的问题。Elabscience®自主开发的新技术,旨在帮助您以更高效的方式进行科学研究。 |
用途 | 试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中IL-17A浓度 |
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-17A抗体包被于酶标板上,实验时样品(或标准品)中的小鼠IL-17A会与包被抗体结合。后依次加入生物素化的抗小鼠IL-17A抗体和辣根过氧化物酶标记的亲和素,抗小鼠IL-17A抗体与结合在包被抗体上的小鼠IL-17A结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IL-17A浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-17A的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | Colormetric |
检测范围 | 7.81-500 pg/mL |
灵敏度 | 2.19 pg/mL |
样本体积 | 25μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的小鼠IL-17A,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Arsenic trioxide improves Treg and Th17 balance by modulating STAT3 in treatment-naïve rheumatoid arthritis patients
IF: 4.932Journal:INTERNATIONAL IMMUNOPHARMACOLOGY -
Fermented Morinda citrifolia (Noni) Alleviates DNCB-Induced Atopic Dermatitis in NC/Nga Mice through Modulating Immune Balance and Skin Barrier Function
IF: 4.546 -
Effect of Perillaldehyde on Prophylaxis and Treatment of Vaginal Candidiasis in a Murine Model
IF: 4.259 -
Preventive and synbiotic effects of the soluble dietary fiber obtained from Lentinula edodes byproducts and Lactobacillus plantarum LP90 against dextran sulfate sodium-induced colitis in mice
IF: 4.125 -
Melatonin attenuates prostatic inflammation and pelvic pain via Sirt1-dependent inhibition of the NLRP3 inflammasome in an EAP mouse model
IF: 4.104 -
4-Methylumbelliferone treatment and hyaluronan inhibition as a therapeutic strategy for chronic prostatitis
IF: 4.104 -
Protective Effect of Prunus mume Fermented with Mixed Lactic Acid Bacteria in Dextran Sodium Sulfate-Induced Colitis
IF: 4.092 -
IL-17 upregulates MCP-1 expression via Act1 / TRAF6 / TAK1 in experimental autoimmune myocarditis
IF: 3.861 -
A sesquiterpene isolated from the stems and leaves of Dioscorea opposita thunb. Transforms the composition of immune cells through ERβ in a mouse model of LPS-induced lung injury
IF: 3.776Journal:Heliyon -
Therapeutic effects of anti–B7-H3 antibody in an ovalbumin-induced mouse asthma model
IF: 3.449Journal:ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY
E-EL-M0047 | Mouse IL-17A(Interleukin 17A) ELISA Kit | 96T , 48T , 96T*5 |