MS Mouse IL-4(Interleukin 4) ELISA Kit
中文名称:MS-小鼠白介素4(IL-4)酶联免疫吸附测定试剂盒
别称:
IL4、BCGF-1、BCGF1、BSF-1、BSF1
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 15.63-1000 pg/mL
- 灵敏度: 9.38 pg/mL
系列介绍 | MS为Mini Sample的缩写。MS系列试剂盒的上样量仅为传统夹心法ELISA试剂盒的1/4,能有效解决实验样本体积不够的问题。Elabscience®自主开发的新技术,旨在帮助您以更高效的方式进行科学研究。 |
用途 | 试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中IL-4浓度 |
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-4抗体包被于酶标板上,实验时样品(或标准品)中的小鼠IL-4会与包被抗体结合。后依次加入生物素化的抗小鼠IL-4抗体和辣根过氧化物酶标记的亲和素,抗小鼠IL-4抗体与结合在包被抗体上的小鼠IL-4结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IL-4浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-4的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | Colormetric |
检测范围 | 15.63-1000 pg/mL |
灵敏度 | 9.38 pg/mL |
样本体积 | 25μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的小鼠IL-4,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Immunogenicity of the recombinant HxuCBA proteins encoded by hxuCBA gene cluster of Haemophilus parasuis in mice
IF: 3.688Journal:GENE -
DHCR24 overexpression modulates microglia polarization and inflammatory response via Akt/GSK3β signaling in Aβ25–35 treated BV-2 cells
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Effects of iron chelating agent on Schistosoma mansoni infected murine model
IF: 3.457 -
Therapeutic effects of anti–B7-H3 antibody in an ovalbumin-induced mouse asthma model
IF: 3.449Journal:ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY -
DPP4 Inhibitor Sitagliptin Reduces Inflammatory Responses and Mast Cell Activation in Allergic Rhinitis
IF: 3.429Journal:PHARMACOLOGY -
Molecular and functional characterization of HtrA protein in Actinobacillus pleuropneumoniae
IF: 3.293 -
Structural characterization of two endopolysaccharides from Phellinus sp. and their immunologic effects by intragastric administration in a healthy mammalian model
IF: 3.289Journal:Food & Function -
Glucosylceramide synthase regulates hepatocyte repair after concanavalin A-induced immune-mediated liver injury
IF: 2.984 -
Effect of xanthohumol on Th1/Th2 balance in a breast cancer mouse model
IF: 2.976Journal:ONCOLOGY REPORTS -
BCG stimulation promotes dendritic cell proliferation and expression of VDR and CYP27B1 in vitamin D‑deficient mice
IF: 2.952Journal:Molecular Medicine Reports
E-EL-M0043 | Mouse IL-4(Interleukin 4) ELISA Kit | 96T , 48T , 96T*5 |