MS Mouse LEP(Leptin) ELISA Kit
中文名称:MS-小鼠瘦素(LEP)酶联免疫吸附测定试剂盒
别称:
LEPD、OB、OBS、Obesity Homolog
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 0.63-40 ng/mL
- 灵敏度: 0.38 ng/mL
系列介绍 | MS为Mini Sample的缩写。MS系列试剂盒的上样量仅为传统夹心法ELISA试剂盒的1/4,能有效解决实验样本体积不够的问题。Elabscience®自主开发的新技术,旨在帮助您以更高效的方式进行科学研究。 |
用途 | 试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中LEP浓度 |
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠LEP抗体包被于酶标板上,实验时样品(或标准品)中的小鼠LEP会与包被抗体结合。后依次加入生物素化的抗小鼠LEP抗体和辣根过氧化物酶标记的亲和素,抗小鼠LEP抗体与结合在包被抗体上的小鼠LEP结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,LEP浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中LEP的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | Colormetric |
检测范围 | 0.63-40 ng/mL |
灵敏度 | 0.38 ng/mL |
样本体积 | 25μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的小鼠LEP,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Effects of incremental endosulfan sulfate exposure and high fat diet on lipid metabolism, glucose homeostasis and gut microbiota in mice
IF: 6.793 -
Differential Responses of White Adipose Tissue and Brown Adipose Tissue to Calorie Restriction During Aging
IF: 6.053Journal:JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCESPMID:32222773 -
Procyanidin B2 ameliorates free fatty acids-induced hepatic steatosis through regulating TFEB-mediated lysosomal pathway and redox state
IF: 6.02Journal:FREE RADICAL BIOLOGY AND MEDICINE -
Bifidobacterium adolescentis Isolated from Different Hosts Modifies the Intestinal Microbiota and Displays Differential Metabolic and Immunomodulatory Properties in Mice Fed a High-Fat Diet
IF: 5.719 -
Neonicotinoid insecticides exposure cause amino acid metabolism disorders, lipid accumulation and oxidative stress in ICR mice
IF: 5.108 -
B-Cell-Activating Factor Depletion Ameliorates Aging-Dependent Insulin Resistance via Enhancement of Thermogenesis in Adipose Tissues
IF: 4.556Journal:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES -
A multi-targeting strategy to ameliorate high-fat-diet- and fructose-induced (western diet-induced) non-alcoholic fatty liver disease (NAFLD) with supplementation of a mixture of legume ethanol extracts
IF: 4.171 -
Hypothermic Effect of Acute Citral Treatment during LPS-induced Systemic Inflammation in Obese Mice: Reduction of Serum TNF-α and Leptin Levels
IF: 4.082 -
Asprosin, a novel glucogenic adipokine: a potential therapeutic implication in diabetes mellitus
IF: 4.076 -
Berberine attenuated olanzapine-induced metabolic alterations in mice: Targeting transient receptor potential vanilloid type 1 and 3 channels
IF: 3.647Journal:LIFE SCIENCES
E-EL-M3008 | Mouse LEP(Leptin) ELISA Kit | 96T , 48T , 96T*5 |