One-step TUNEL Flow Cytometry Apoptosis Kit (Blue, Elab Fluor® Violet 450)
中文名称:一步法TUNEL流式凋亡检测试剂盒(蓝色,Elab Fluor® Violet 450)
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- 应用: 细胞凋亡-DNA片段化
检测原理 | 细胞在发生凋亡时,会激活一些特异性的DNA内切酶,这些内切酶会切断核小体间的基因组DNA。使得凋亡细胞的DNA被切割成180~200bp片段,在琼脂糖凝胶上通常以180~200bp的阶梯状迁移。TdT酶(脱氧核糖核酸末端转移酶)将标记的dUTP连接到断裂DNA暴露的3'-OH末端,通过在这些末端添加荧光dUTP的方式来标记晚期凋亡细胞,从而可以通过流式细胞仪进行检测。 |
检测方法 | 荧光;流式 |
样本类型 | 细胞 |
检测时间 | 2.5-3 hours |
检测仪器 | 流式细胞仪 |
荧光 | Elab Fluor®Violet 450 |
Ex/Em | 410/450 |
Channel set | Pacific Blue |
自备试剂 | PBS(含1%BSA)缓冲液(pH7.2~7.4) |
保存温度 | 该产品可在-20°C避光条件下保存12个月。 |
有效期 | 12个月 |
运输条件 | 冰袋 |
E-CK-A233 | Annexin V-Elab Fluor® Violet 450/PI Apoptosis Kit | 200Assays , 100Assays , 50Assays , 20Assays |
E-CK-A301 | Mitochondrial Membrane Potential Assay Kit (with JC-1) | 100Assays , 50Assays , 20Assays |
E-CK-A362 | Enhanced Cell Counting Kit 8 (WST-8/CCK8) | 500Tests , 100Tests , 500Tests×20 |
Q1:Can TUNEL assay kit be used for mouse samples?
There is no species limination in TUNEL assay kits.
Q2:Can the One-step TUNEL kit detect a sperm sample?
Yes, it can be used to test sperm samples.
Q3:What is the difference between TUNEL in-situ kit and TUNEL flow cytometry kit?
The main difference lies in the detection methods, for TUNEL in-situ kits, a fluorescence microscope is used for observing the results, as for TUNEL flow ytometry kit, the sample will be detected by a flow cytometer. On the other hand, TUNEL in-situ kit is more suitable for tissue sections and cell slides or smears, while TUNEL flow ytometry kit is more suitable for suspended cells and adherent cells.
Q4:Is there any TUNEL assay kit that can detect apoptosis in erythrocyte?
The detection of TUNEL is based on DNA fragmentation in late stage apoptosis, so it is not avaliable for erythrocyte as there is no nuclei or other organelles containing DNA in erythrocyte.
Q5:What are the reasons that lead to false positives in TUNEL?
Cells with high proliferative or metabolic active may have a break in the DNA strand, both cases may end up with false-positive result. Evaluation of cell morphology may be used if there are doubts about the interpretation of the results.