Enhanced Cell Counting Kit 8 (WST-8/CCK8)
中文名称:增强型细胞活力检测试剂盒(CCK-8)
Price:
- 应用: 细胞活力/细胞增殖/细胞毒性
检测原理 | WST-8 (2-(2-甲氧基-4-硝苯基)-3-(4-硝苯基)-5-(2,4-二磺基苯)-2H-四唑单钠盐),在电子载体存在的情况下,WST-8被线粒体内的脱氢酶还原生成水溶性的橙黄色formazan,生成的formazan的量与活细胞数量成正比,通过检测在450 nm处的吸光度,可以间接计算活细胞的量。 |
检测方法 | 比色法 |
样本类型 | 细胞 |
检测仪器 | 酶标仪 |
保存温度 | 该产品可在 2~8°C或-20°C避光条件下保存24个月。 |
有效期 | 24个月 |
运输条件 | 冰袋 |
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E-CK-A211 | Annexin V-FITC/PI Apoptosis Kit | 200Assays , 100Assays , 50Assays , 20Assays |
E-CK-A301 | Mitochondrial Membrane Potential Assay Kit (with JC-1) | 100Assays , 50Assays , 20Assays |
E-CK-A320 | One-step TUNEL In Situ Apoptosis Kit (Green, FITC) | 100Assays , 50Assays , 20Assays |
E-CK-A341 | MTT Cell Proliferation and Cytotoxicity Assay Kit | 1000Assays , 500Assays |
Q1:请问CCK8和凋亡的摸出来的药物IC50不一样的原因。
IC50指的是半抑制率,可以用CCK8检测,凋亡实验检测的是凋亡率,这个不等同于IC50。这两个实验的实验目的是不一样的。
Q2:用来刺激细胞的药物有颜色,会影响实验结果吗?
可以在药物孵育结束后,加入CCK-8之前更换培养基,以此来消除药物颜色的影响。
Q3:MTS, XXT, WST-1, CCK-8(WST-8)检测试剂盒的差异?
MTT检测试剂盒广泛用于检测细胞增殖和细胞毒性,而MTS, XXT, WST-1, WST-8都是MTT的升级替代产品,和MTT相比有明显优点。首先,MTT被线粒体内的一些脱氢酶还原成生成的formazan不是水溶性的,需要有特定的溶剂来溶解。WST-8和XTT,MTS产生的formazan都是水溶性的,可以省去后续的溶解步骤。其次,WST-8产生的formazan比XTT,MTS产生的formazan更易溶解。最后,WST-8比MTT,XTT更稳定,线性范围更宽,灵敏度更高。
Q4:CCK-8试剂盒的检测分辨率
CCK-8试剂盒的OD值在0.2~2.5之间具有良好的线性关系
Q5:CCK-8的最佳OD值范围是多少呢?
一般CCK-8的吸光度值建议在1左右,最好是0.8~1.2。
Q6:CCK-8建议用哪种类型的96孔板?
建议用透光的平底,且独立柱状相对最适合。