Rat HMGB-1(High Mobility Group Protein B1) ELISA Kit
中文名称:大鼠高迁移率族蛋白B1(HMGB-1)酶联免疫吸附测定试剂盒
别称:
HMGB1、HMG1、HMG3、SBP-1、Amphoterin
Price:
Size:
96T
48T
96T*5
- 反应性: Rat
- 检测范围: 31.25-2000 pg/mL
- 灵敏度: 18.75 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠HMGB-1抗体包被于酶标板上,实验时样品(或标准品)中的大鼠HMGB-1会与包被抗体结合。后依次加入生物素化的抗大鼠HMGB-1抗体和辣根过氧化物酶标记的亲和素,抗大鼠HMGB-1抗体与结合在包被抗体上的大鼠HMGB-1结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,HMGB-1浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中HMGB-1的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Rat |
检测方法 | Colormetric |
检测范围 | 31.25-2000 pg/mL |
灵敏度 | 18.75 pg/mL |
样本体积 | 100μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的大鼠HMGB-1,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Azilsartan Modulates HMGB1/NF-κB/p38/ERK1/2/JNK and Apoptosis Pathways during Renal Ischemia Reperfusion Injury
IF: 7.666Journal:Cells -
Irbesartan reprofiling for the amelioration of ethanol-induced gastric mucosal injury in rats: Role of inflammation, apoptosis, and autophagy
IF: 6.78 -
The Role of IGL-2 Preservation Solution on Rat Livers during SCS and HOPE
IF: 6.208 -
Normothermic machine perfusion attenuates hepatic ischaemia-reperfusion injury by inhibiting CIRP-mediated oxidative stress and mitochondrial fission
IF: 5.31 -
Repositioning Linagliptin for the Mitigation of Cadmium-Induced Testicular Dysfunction in Rats: Targeting HMGB1/TLR4/NLRP3 Axis and Autophagy
IF: 5.215 -
Exploring the Mechanism on the Medullary Visceral Zone Inhibiting the Cholinergic Anti-inflammatory Pathway Induced by Sepsis
IF: 4.711 -
Oleoylethanolamide prevents neuroimmune HMGB1/TLR4/NF‐kB danger signaling in rat frontal cortex and depressive‐like behavior induced by ethanol binge administration
IF: 4.547Journal:ADDICTION BIOLOGY -
Protective effect of glycyrrhizin, a direct HMGB1 inhibitor, on post-contrast acute kidney injury
IF: 4.38 -
Prunella vulgaris L. Attenuates Experimental Autoimmune Thyroiditis by Inhibiting HMGB1/TLR9 Signaling
IF: 4.162 -
HMGB1/RAGE pro-inflammatory axis promotes vascular endothelial cell apoptosis in limb ischemia/reperfusion injury
IF: 3.743Journal:BIOMEDICINE & PHARMACOTHERAPY