Rat HMGB-1(High Mobility Group Protein B1) ELISA Kit
中文名称:大鼠高迁移率族蛋白B1(HMGB-1)酶联免疫吸附测定试剂盒
别称:
HMGB1、HMG1、HMG3、SBP-1、Amphoterin
Price:
Size:
96T
48T
96T*5
- 反应性: Rat
- 检测范围: 31.25-2000 pg/mL
- 灵敏度: 18.75 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠HMGB-1抗体包被于酶标板上,实验时样品(或标准品)中的大鼠HMGB-1会与包被抗体结合。后依次加入生物素化的抗大鼠HMGB-1抗体和辣根过氧化物酶标记的亲和素,抗大鼠HMGB-1抗体与结合在包被抗体上的大鼠HMGB-1结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,HMGB-1浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中HMGB-1的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Rat |
检测方法 | Colormetric |
检测范围 | 31.25-2000 pg/mL |
灵敏度 | 18.75 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的大鼠HMGB-1,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Ethanol directly induced HMGB1 release through NOX2/NLRP1 inflammasome in neuronal cells
IF: 3.621Journal:TOXICOLOGY -
HMGB1 is mechanistically essential in the development of experimental pulmonary hypertension
IF: 3.553 -
Impact of uric acid on liver injury and intestinal permeability following resuscitated hemorrhagic shock in rats
IF: 3.381 -
PDGFR-β signaling mediates HMGB1 release in mechanically stressed vascular smooth muscle cells
IF: 3.24 -
Glycyrrhizin potentially suppresses the inflammatory response in preeclampsia rat model
IF: 2.095Journal:Pregnancy Hypertension-An International Journal of Womens Cardiovascular Health -
Carbon Monoxide Inhibits the Expression of Proteins Associated with Intestinal Mucosal Pyroptosis in a Rat Model of Sepsis Induced by Cecal Ligation and Puncture
IF: 1.918Journal:MEDICAL SCIENCE MONITOR -
Human umbilical cord‑derived mesenchymal stem cells and human cord blood mononuclear cells protect against cisplatin‑induced acute kidney injury in rat models
IF: 1.785Journal:Experimental and Therapeutic Medicine -
Sivelestat sodium hydrate improves post‑traumatic knee osteoarthritis through nuclear factor‑κB in a rat model
IF: 1.261Journal:Experimental and Therapeutic Medicine -
Use of a high platelet-to-RBC ratio of 2:1 is more effective in correcting trauma-induced coagulopathy than a ratio of 1:1 in a rat multiple trauma transfusion model
Journal:Intensive Care Medicine Experimental