Rat HO1(Heme Oxygenase 1) ELISA Kit
中文名称:大鼠血红素氧合酶1(HO1)酶联免疫吸附测定试剂盒
别称:
HO-1、HMOX1、HMOX1D、HSP32、Bk286b10
Price:
Size:
96T
48T
96T*5
- 反应性: Rat
- 检测范围: 0.16-10 ng/mL
- 灵敏度: 0.1 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠HO1抗体包被于酶标板上,实验时样品(或标准品)中的大鼠HO1会与包被抗体结合。后依次加入生物素化的抗大鼠HO1抗体和辣根过氧化物酶标记的亲和素,抗大鼠HO1抗体与结合在包被抗体上的大鼠HO1结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,HO1浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中HO1的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Rat |
检测方法 | Colormetric |
检测范围 | 0.16-10 ng/mL |
灵敏度 | 0.1 ng/mL |
样本体积 | 100μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的大鼠HO1,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Carveol Promotes Nrf2 Contribution in Depressive Disorders through an Anti-inflammatory Mechanism
IF: 6.543 -
Serum metabolomics analysis reveals amelioration effects of sea cucumber ether phospholipids on oxidative stress and inflammation in high-fat diet-fed mice
IF: 6.317 -
Origanum majorana L. Extract Attenuated Benign Prostatic Hyperplasia in Rat Model: Effect on Oxidative Stress, Apoptosis, and Proliferation
IF: 6.313 -
Carveol a Naturally-Derived Potent and Emerging Nrf2 Activator Protects Against Acetaminophen-Induced Hepatotoxicity
IF: 5.811 -
Banana peel ameliorated hepato-renal damage and exerted anti-inflammatory and anti-apoptotic effects in metal mixture mediated hepatic nephropathy by activation of Nrf2/ Hmox-1 and inhibition of Nfkb pathway
IF: 5.572 -
Zinc and selenium mitigated heavy metals mixture (Pb, Al, Hg and Mn) mediated hepatic-nephropathy via modulation of oxido-inflammatory status and NF‑kB signaling in female albino rats
IF: 4.571 -
Dimethyl fumarate attenuates 2-VO-induced vascular dementia via activating the Nrf2 signaling pathway in rats
IF: 4.473 -
Activation of the Nrf2/HO-1 signaling pathway by dimethyl fumarate ameliorates complete Freund's adjuvant-induced arthritis in rats
IF: 4.432 -
Zinc oxide nanoparticle-induced atherosclerotic alterations in vitro and in vivo
IF: 4.3Journal:International Journal of Nanomedicine -
Doxorubicin-induced Hepatic Toxicity in Rats: Mechanistic Protective Role of Omega-3 Fatty Acids through Nrf2/HO-1 activation and PI3K/Akt/GSK-3β Axis Modulation
IF: 4.219