Rat HO1(Heme Oxygenase 1) ELISA Kit
中文名称:大鼠血红素氧合酶1(HO1)酶联免疫吸附测定试剂盒
别称:
HO-1、HMOX1、HMOX1D、HSP32、Bk286b10
Price:
Size:
96T
48T
96T*5
- 反应性: Rat
- 检测范围: 0.16-10 ng/mL
- 灵敏度: 0.1 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠HO1抗体包被于酶标板上,实验时样品(或标准品)中的大鼠HO1会与包被抗体结合。后依次加入生物素化的抗大鼠HO1抗体和辣根过氧化物酶标记的亲和素,抗大鼠HO1抗体与结合在包被抗体上的大鼠HO1结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,HO1浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中HO1的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Rat |
检测方法 | Colormetric |
检测范围 | 0.16-10 ng/mL |
灵敏度 | 0.1 ng/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的大鼠HO1,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Nrf2/HO-1 Signaling Stimulation through Acetyl-11-Keto-Beta-Boswellic Acid (AKBA) Provides Neuroprotection in Ethidium Bromide-Induced Experimental Model of Multiple Sclerosis
IF: 4.141 -
Activation of pCREB/Nrf-2 signaling mediates re-positioning of liraglutide as hepato-protective for methotrexate -induced liver injury (MILI)
IF: 3.775 -
Essential Trace Elements Prevent the Impairment in the Retention Memory, Cerebral Cortex, and Cerebellum Damage in Male Rats Exposed to Quaternary Metal Mixture by Up-regulation, of Heme Oxygynase-1 and Down-regulation of Nuclear Factor Erythroid 2-relate
IF: 3.708 -
Potent Natural Antioxidant Carveol Attenuates MCAO-Stress Induced Oxidative, Neurodegeneration by Regulating the Nrf-2 Pathway
IF: 3.707Journal:Frontiers in Neuroscience -
New insights into the protection of growth hormone in cisplatin-induced nephrotoxicity: The impact of IGF-1 on the Keap1-Nrf2/HO-1 signaling
IF: 3.647 -
The interrelationship between gasotransmitters and lead-induced renal toxicity in rats
IF: 3.499Journal:TOXICOLOGY LETTERS -
Octreotide alleviates pancreatic damage caused by paraquat in rats by reducing inflammatory responses and oxidative stress
IF: 3.292 -
Effects of sulforaphane on D-galactose-induced liver aging in rats: Role of keap-1/nrf-2 pathway.
IF: 3.17Journal:EUROPEAN JOURNAL OF PHARMACOLOGY -
The potential relationship between gasotransmitters and oxidative stress, inflammation and apoptosis in lead-induced hepatotoxicity in rats
IF: 2.466 -
Transferring the protective effect of remote ischemic preconditioning on skin flap among rats by blood serum
IF: 1.037