Rat INS(Insulin) ELISA Kit
中文名称:大鼠胰岛素(INS)酶联免疫吸附测定试剂盒
别称:
IDDM2、ILPR、IRDN、MODY10
Price:
Size:
96T
48T
96T*5
- 反应性: Rat
- 检测范围: 6.25-400 pg/mL
- 灵敏度: 3.75 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠INS抗体包被于酶标板上,实验时样品(或标准品)中的大鼠INS会与包被抗体结合。后依次加入生物素化的抗大鼠INS抗体和辣根过氧化物酶标记的亲和素,抗大鼠INS抗体与结合在包被抗体上的大鼠INS结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,INS浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中INS的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Rat |
检测方法 | Colormetric |
检测范围 | 6.25-400 pg/mL |
灵敏度 | 3.75 pg/mL |
样本体积 | 100μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的大鼠INS,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Kelulut Honey Ameliorates Oestrus Cycle, Hormonal Profiles, and Oxidative Stress in Letrozole-Induced Polycystic Ovary Syndrome Rats
IF: 7.675 -
Therapeutic Effects of Heterotrigona itama (Stingless Bee) Bee Bread in Improving Hepatic Lipid Metabolism through theActivation of the Keap1/Nrf2 Signaling Pathway in an Obese Rat Model
IF: 7.675Journal:Antioxidants -
Lycium barbarum polysaccharide combined with aerobic exercise ameliorated nonalcoholic fatty liver disease through restoring gut microbiota, intestinal barrier and inhibiting hepatic inflammation
IF: 6.953Journal:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULESPMID:33992651 -
Curcumin in Combination with Aerobic Exercise Improves Follicular Dysfunction via Inhibition of the Hyperandrogen-Induced IRE1α/XBP1 Endoplasmic Reticulum Stress Pathway in PCOS-Like Rats
IF: 6.543Journal:Oxidative Medicine and Cellular Longevity -
Diacerein ameliorates letrozole-induced polycystic ovarian syndrome in rats
IF: 6.53 -
D-ribose-L-cysteine prevents oxidative stress and cardiometabolic syndrome in high fructose high fat diet fed rats
IF: 6.53 -
Acrylamide induced glucose metabolism disorder in rats involves gut microbiota dysbiosis and changed bile acids metabolism
IF: 6.475 -
Development and Evaluation of Curcumin Encapsulated Self-assembled Nanoparticles as Potential Remedial Treatment for PCOS in a Female Rat Model
IF: 6.4 -
Grafting Resveratrol onto Mesoporous Silica Nanoparticles towards Efficient Sustainable Immunoregulation and Insulin Resistance Alleviation for Diabetic Periodontitis Therapy
IF: 6.331 -
Conjugated linoleic acid alleviates glycolipid metabolic disorders by modulating intestinal microbiota and short-chain fatty acids in obese rats
IF: 6.317Journal:Food & Function