TGF-β1(Transforming Growth Factor Beta 1) ELISA Kit
中文名称:转化生长因子β1(TGF-β1)酶联免疫吸附测定试剂盒
Cartilage-inducing factor、CED、Differentiation inhibiting factor、DPD1、LAP、Latency-associated peptide、Prepro transforming growth factor beta 1、TGF beta 1、TGF beta、TGF beta 1 protein、TGF-beta 1 protein、TGF-beta-1、TGF-beta-5、TGF-beta1、TGFB、Tgfb-1、Tgfb1、TGFB1、TGFbeta、TGFbeta1、Transforming Growth Factor b1、Transforming Growth Factor beta 1、Transforming growth factor beta 1a、Transforming growth factor beta-1、Transforming growth factor、Beta 1、Transforming Growth Factor-1
Price:
- 反应性: Universal
- 检测范围: 0.16-10 ng/mL
- 灵敏度: 0.1 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗TGF-β1抗体包被于酶标板上,实验时样品(或标准品)中的TGF-β1会与包被抗体结合。后依次加入生物素化的抗TGF-β1抗体和辣根过氧化物酶标记的亲和素,抗TGF-β1抗体与结合在包被抗体上的TGF-β1结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,TGF-β1浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中TGF-β1的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Universal |
检测方法 | Colormetric |
检测范围 | 0.16-10 ng/mL |
灵敏度 | 0.1 ng/mL |
样本体积 | 100μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的TGF-β1,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
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Q1: Why tissue samples do not need to be activated when TGF- beta (transforming growth factor - beta) was detected;
Since the antibodies in the kit recognize activated TGF-β, and TGF-β is a disulfide bond linked by two identical or similar 12.5kDa subunits of molecular weight. The study of human TGF-β cDNA sequence showed that the 112 amino acid residues of the monomer TGF-β were cleaved from the carboxyl terminal by a precursor molecule (per-pro-TGF-β) containing 400 amino acid residues. The N-terminal of per-pro-TGF-β contains a signal peptide, which is cleaved before secretion to become an inactive polypeptide chain precursor (pro-TGF-β). The n-terminal part of the amino acid residue is removed by changing the ionic strength, acidification or protease hydrolysis, and the remaining carboxyl terminal part forms an active TGF-β. A variety of cells in the body can secrete TGF-β in an inactive state. In vitro, the inactive TGF-β, also known as latency associated peptide (LAP), can be activated by acidification. In vivo, the acidic environment can be present near fractures and healing wounds, and the cleavage of the protein itself can cause the TGF-β complex to become activated TGF-β. In general, tissues with active cell differentiation often contain high levels of TGF-β, such as osteoblasts, kidney, bone marrow, and fetal liver hematopoietic cells. Therefore, no additional activation processing is required for tissue sample testing.