TGF-β1(Transforming Growth Factor Beta 1) ELISA Kit
中文名称:转化生长因子β1(TGF-β1)酶联免疫吸附测定试剂盒
Cartilage-inducing factor、CED、Differentiation inhibiting factor、DPD1、LAP、Latency-associated peptide、Prepro transforming growth factor beta 1、TGF beta 1、TGF beta、TGF beta 1 protein、TGF-beta 1 protein、TGF-beta-1、TGF-beta-5、TGF-beta1、TGFB、Tgfb-1、Tgfb1、TGFB1、TGFbeta、TGFbeta1、Transforming Growth Factor b1、Transforming Growth Factor beta 1、Transforming growth factor beta 1a、Transforming growth factor beta-1、Transforming growth factor、Beta 1、Transforming Growth Factor-1
Price:
- 反应性: Universal
- 检测范围: 0.16-10 ng/mL
- 灵敏度: 0.1 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗TGF-β1抗体包被于酶标板上,实验时样品(或标准品)中的TGF-β1会与包被抗体结合。后依次加入生物素化的抗TGF-β1抗体和辣根过氧化物酶标记的亲和素,抗TGF-β1抗体与结合在包被抗体上的TGF-β1结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,TGF-β1浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中TGF-β1的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Universal |
检测方法 | Colormetric |
检测范围 | 0.16-10 ng/mL |
灵敏度 | 0.1 ng/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的TGF-β1,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Intragastric administration of Pien Tze Huang enhanced wound healing in diabetes by inhibiting inflammation and improving energy generation
IF: 6.656Journal:PHYTOMEDICINE -
A collagen scaffold loaded with human umbilical cord-derived mesenchymal stem cells facilitates endometrial regeneration and restores fertility
IF: 6.638 -
DNA Methyltransferase 3b Accelerates the Process of Atherosclerosis
IF: 6.543 -
L-ergothioneine and its combination with metformin attenuates renal dysfunction in type-2 diabetic rat model by activating Nrf2 antioxidant pathway
IF: 6.53 -
Indirubin regulates T cell differentiation by promoting αVβ8 expression in bone marrow-derived dendritic cells to alleviate inflammatory bowel disease
IF: 6.388 -
Combination of graphene oxide and platelet-rich plasma improves tendon–bone healing in a rabbit model of supraspinatus tendon reconstruction
IF: 6.353Journal:Regenerative Biomaterials -
Whole grain germinated brown rice regulates intestinal immune homeostasis and gastrointestinal hormones in type 2 diabetic patients—a randomized control trial
IF: 6.317Journal:Food & Function -
Silica particles disorganize the polarization of pulmonary macrophages in mice
IF: 6.291 -
Adamantane-linked isothiourea derivatives suppress the growth of experimental hepatocellular carcinoma via inhibition of TLR4-MyD88-NF-κB signaling
IF: 6.166Journal:American Journal of Cancer ResearchPMID:33575076 -
Retinol-Binding Protein 4 Accelerates Metastatic Spread and Increases Impairment of Blood Flow in Mouse Mammary Gland Tumors
IF: 6.126Journal:Cancers