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当前位置:首页>>细胞功能检测试剂>>细胞凋亡检测>>Annexin V凋亡检测>>试剂盒>> Annexin V-FITC/PI Apoptosis Kit

Annexin V-FITC/PI Apoptosis Kit

文献(120)

  • 中文名称:Annexin V-FITC / PI细胞凋亡检测试剂盒

  • 货号:E-CK-A211

Applications:

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规格:
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价格: ￥450
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检测原理

Annexin V是一种钙离子依赖性磷脂结合蛋白，与磷脂酰丝氨酸PS有高度亲和力，可通过细胞外侧暴露的PS与凋亡早期细胞胞膜结合，将Annexin V标记荧光染料，利用流式细胞仪检测细胞凋亡。 细胞核染料（PI,DAPI,7-AAD）可与双链DNA特异性结合，并产生强烈的荧光，正常情况下无法透过细胞膜。由于凋亡晚期或坏死细胞膜丧失完整性，细胞核染料（DAPI、PI、7-AAD）可进入细胞内对DNA进行染色，与Annexin V搭配使用，可区分处于不同凋亡时期的细胞。

基本信息

应用类型 细胞凋亡-Annexin V
检测方法 荧光
样本类型 细胞
检测时间 40min
检测仪器 流式细胞仪
荧光 FITC
Ex/Em (nm) 490/530
Channel set FITC
自备试剂 PBS(E-BC-R187), 去离子水
保存温度 2~8°C, shading light
运输条件 冰袋
保质期 12 months

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    DOI: 10.1007/s00280-020-04083-3
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Verified Customer

c**qSubmitted [ Dec 04 2019 ]

  • Application:FCM
  • Incubation:Undiluted

    15minutes

  • Description:I like this produce ,because Separating apoptotic cells from normal cells is good. I get the results well.
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