本公司产品仅供体外研究使用,不用于临床诊断

Annexin V-FITC/PI Apoptosis Kit

  • 中文名称:Annexin V-FITC / PI细胞凋亡检测试剂盒

  • 货号:E-CK-A211

订购 E-CK-A211

规格:
  • 20Assays
  • 50Assays
  • 100Assays
  • 200Assays
价格: ¥450
数量:

检测原理

Annexin V是一种钙离子依赖性磷脂结合蛋白,与磷脂酰丝氨酸PS有高度亲和力,可通过细胞外侧暴露的PS与凋亡早期细胞胞膜结合,将Annexin V标记荧光染料,利用流式细胞仪检测细胞凋亡。 细胞核染料(PI,DAPI,7-AAD)可与双链DNA特异性结合,并产生强烈的荧光,正常情况下无法透过细胞膜。由于凋亡晚期或坏死细胞膜丧失完整性,细胞核染料(DAPI、PI、7-AAD)可进入细胞内对DNA进行染色,与Annexin V搭配使用,可区分处于不同凋亡时期的细胞。

基本信息

应用类型 细胞凋亡-Annexin V
检测方法 荧光
样本类型 细胞
检测时间 40min
检测仪器 流式细胞仪
荧光 FITC
Ex/Em (nm) 490/530
Channel set FITC
自备试剂 PBS(E-BC-R187), 去离子水
保存温度 2~8°C, shading light
运输条件 冰袋
保质期 12 months

文献

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  96. CELL CYCLE (2020) IF: 3.699
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  97. Frontiers in Neuroscience (2020) IF: 3.707
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  99. BIOMEDICINE & PHARMACOTHERAPY (2019) IF: 3.743
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  101. MOLECULAR AND CELLULAR BIOCHEMISTRY (2022) IF: 3.842
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    DOI: 10.1016/j.micpath.2022.105964

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    Promoting effects of MiR-135b on human multiple myeloma cells via regulation of the Wnt/β-catenin/Versican signaling pathway

    DOI: 10.1016/j.cyto.2021.155495

    PMID: 33765653

    Sample: Cell sample
  104. EXPERIMENTAL CELL RESEARCH (2022) IF: 3.905
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    DOI: 10.1016/j.yexcr.2022.113181

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  113. Cancer Management and Research (2020) IF: 2.886
    CircSEC31A Promotes the Malignant Progression of Non-Small Cell Lung Cancer Through Regulating SEC31A Expression via Sponging miR-376a

    DOI: 10.2147/CMAR.S280124

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    DOI: 10.3390/medicina58030355

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    DOI: 10.3892/mmr.2021.12418

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    DOI: 10.1007/s00280-020-04083-3

    Sample: Cell lysate
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  119. Genes & Genomics (2022) IF: 1.839
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Verified Customer

c**qSubmitted [ Dec 04 2019 ]

  • Application:FCM
  • Incubation:Undiluted

    15minutes

  • Description:I like this produce ,because Separating apoptotic cells from normal cells is good. I get the results well.
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