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Annexin V-FITC/PI Apoptosis Kit

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中文名称:Annexin V-FITC / PI细胞凋亡检测试剂盒
货号:E-CK-A211

Applications:

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检测原理

Annexin V是一种钙离子依赖性磷脂结合蛋白，与磷脂酰丝氨酸PS有高度亲和力，可通过细胞外侧暴露的PS与凋亡早期细胞胞膜结合，将Annexin V标记荧光染料，利用流式细胞仪检测细胞凋亡。细胞核染料（PI,DAPI,7-AAD）可与双链DNA特异性结合，并产生强烈的荧光，正常情况下无法透过细胞膜。由于凋亡晚期或坏死细胞膜丧失完整性，DAPI可进入细胞内对DNA进行染色，与Annexin V搭配使用，可区分处于不同凋亡时期的细胞。

基本信息

应用类型	细胞凋亡-Annexin V
检测方法	荧光
样本类型	细胞
检测时间	40min
检测仪器	流式细胞仪
荧光	FITC
Ex/Em (nm)	490/530
Channel set	FITC
自备试剂	PBS(E-BC-R187), 去离子水
保存温度	2~8°C, shading light
运输条件	冰袋
保质期	12 months

文献

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Verified Customer

c**qSubmitted [ Dec 04 2019 ]

Application:FCM
Incubation:Undiluted
15minutes
Description:I like this produce ,because Separating apoptotic cells from normal cells is good. I get the results well.

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E-CK-A151	Annexin V Binding Buffer (10×)
E-CK-A111	Annexin V-FITC Reagent
E-CK-A212	Annexin V-FITC/7-AAD Apoptosis Kit
E-CK-A252	Annexin V-FITC/DAPI Apoptosis Kit
E-CK-A320	One-step TUNEL In Situ Apoptosis Kit (Green, FITC)
E-CK-A301	Mitochondrial Membrane Potential Assay Kit (with JC-1)
E-CK-A311	Caspase 3 Activity Colorimetric Assay Kit

Annexin V-FITC/PI Apoptosis Kit

订购 E-CK-A211

检测原理

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文献

Aging (Albany NY) (2020) IF: 4.831

Microorganisms (2020) IF: 4.152

Cancer Chemotherapy and Pharmacology (2020) IF: 3.008

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