MTT Cell Proliferation and Cytotoxicity Assay Kit
中文名称：MTT细胞增殖及细胞毒性检测试剂盒
Price:
Size:
1000Assays
500Assays
- 应用： 细胞活力/细胞增殖/细胞毒性
| 检测原理 | MTT是噻唑蓝（3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide），可被线粒体内的一些脱氢酶还原生成结晶状的深紫色产物甲臜（formazan）。甲臜可被DMSO完全溶解，并通过酶标仪测定570nm波长附近的吸光度。细胞增殖速度越快，则吸光度越高；细胞毒性越大，则细胞增殖减慢从而导致吸光度降低。 |
| 检测方法 | 比色法 |
| 样本类型 | 细胞 |
| 检测仪器 | 酶标仪 |
| 自备试剂 | DMSO |
| 保存温度 | 该产品可在 2~8°C避光条件下保存6个月。 |
| 有效期 | 6个月 |
| 运输条件 | 冰袋 |
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| E-CK-A211 | Annexin V-FITC/PI Apoptosis Kit | 200Assays , 100Assays , 50Assays , 20Assays |
| E-CK-A301 | Mitochondrial Membrane Potential Assay Kit (with JC-1) | 100Assays , 50Assays , 20Assays |
| E-CK-A320 | One-step TUNEL In Situ Apoptosis Kit (Green, FITC) | 100Assays , 50Assays , 20Assays |
| E-CK-A362 | Enhanced Cell Counting Kit 8 (WST-8/CCK8) | 500Tests , 100Tests , 500Tests×20 |
Q1:What are the differences among MTS, XXT, WST-1, CCK-8 (WST-8) test kits?
MTT assay kits are widely used to detect cell proliferation and cytotoxicity, and MTS, XXT, WST-1 and WST-8 are all upgraded alternatives to MTT, which have obvious advantages compared with MTT. On the one hand, the reaction production of MTT assay is insoluable in water, a specific solvent is required in the assay, products of WST-8, XTT and MTS are water-soluble with easier operation. On the other hand, the solubility of the formazan produced of WST-8 is higher than that of XTT and MTS. What's more, WST-8 is more stable than MTT, XTT, and has a wider linear range and higher sensitivity.
