Human IL-6(Interleukin 6) ELISA Kit
中文名称:人白介素6(IL-6)酶联免疫吸附测定试剂盒
别称:
IL6、BSF2、HGF、HSF、IFNB2、CDF
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 1.56-100 pg/mL
- 灵敏度: 0.94 pg/mL
用途 | 试剂盒用于体外定量检测人血清、血浆或其他生物体液中IL-6浓度 |
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-6抗体包被于酶标板上,实验时样品或标准品中的人IL-6会与包被抗体结合,游离的成分被洗去。依次加入生物素化的抗人IL-6抗体和辣根过氧化物酶标记的亲和素。抗人IL-6抗体与结合在包被抗体上的人IL-6结合、生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,IL-6浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-6的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | Colormetric |
检测范围 | 1.56-100 pg/mL |
灵敏度 | 0.94 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的人IL-6,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
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Chronic exposure to Cytolethal Distending Toxin (CDT) promotes a cGAS-dependent type I interferon response
IF: 9.261 -
METTL3 inhibits inflammation of retinal pigment epithelium cells by regulating NR2F1 in an m6A-dependent manner
IF: 8.786 -
Red Blood Cells-Derived Vesicles for Delivery of Lipophilic Drug Camptothecin
IF: 8.456Journal:ACS Applied Materials & Interfaces -
Physicochemical properties and protective effects on UVA-induced photoaging in Hs68 cells of Pleurotus ostreatus polysaccharides by fractional precipitation
IF: 8.025Journal:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES -
Rational design of a prodrug to inhibit self-inflammation for cancer treatment
IF: 7.79 -
Golgi Phosphoprotein 3 Mediates Radiation-Induced Bystander Effect via ERK/EGR1/TNF-α Signal Axis
IF: 7.675Journal:Antioxidants -
Genipin Attenuates Diabetic Cognitive Impairment by Reducing Lipid Accumulation and Promoting Mitochondrial Fusion via FABP4/Mfn1 Signaling in Microglia
IF: 7.675Journal:Antioxidants -
TIPE2 May Target the Nrf2/HO-1 Pathway to Inhibit M1 Macrophage-Related Neutrophilic Inflammation in Asthma.
IF: 7.561Journal:Frontiers in Immunology -
Lactobacillus plantarum CCFM1143 Alleviates Chronic Diarrhea via Inflammation Regulation and Gut Microbiota Modulation: A Double-Blind, Randomized, Placebo-Controlled Study
IF: 7.561 -
Predictive value of soluble CD59 for poor 28-day neurological prognosis and all-cause mortality in patients after cardiopulmonary resuscitation: a prospective observatory study
IF: 7.52Journal:Journal of Intensive Care
E-UNEL-H0097 | Uncoated Human IL-6(Interleukin 6) ELISA Kit | 96T*5 , 96T*15 |