Human IL-6(Interleukin 6) ELISA Kit
中文名称:人白介素6(IL-6)酶联免疫吸附测定试剂盒
别称:
IL6、BSF2、HGF、HSF、IFNB2、CDF
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 1.56-100 pg/mL
- 灵敏度: 0.94 pg/mL
用途 | 试剂盒用于体外定量检测人血清、血浆或其他生物体液中IL-6浓度 |
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-6抗体包被于酶标板上,实验时样品或标准品中的人IL-6会与包被抗体结合,游离的成分被洗去。依次加入生物素化的抗人IL-6抗体和辣根过氧化物酶标记的亲和素。抗人IL-6抗体与结合在包被抗体上的人IL-6结合、生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,IL-6浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-6的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | Colormetric |
检测范围 | 1.56-100 pg/mL |
灵敏度 | 0.94 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的人IL-6,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Alteration in Gut Microbiota Associated with Zinc Deficiency in School-Age Children
IF: 6.706 -
Fexofenadine Protects Against Intervertebral Disc Degeneration Through TNF Signaling
IF: 6.684Journal:Frontiers in Cell and Developmental Biology -
EZH2-Inhibited MicroRNA-454-3p Promotes M2 Macrophage Polarization in Glioma
IF: 6.684 -
Magnoflorine attenuates inflammatory responses in RA by regulating the PI3K/Akt/NF-κB and Keap1-Nrf2/HO-1 signalling pathways in vivo and in vitro
IF: 6.656 -
Different oral and gut microbial profiles in those with Alzheimer's disease consuming anti-inflammatory diets.
IF: 6.59Journal:Frontiers in Nutrition -
Autophagic Degradation of Gasdermin D Protects against Nucleus Pulposus Cell Pyroptosis and Retards Intervertebral Disc Degeneration In Vivo
IF: 6.543 -
In Vitro Antibacterial and Anti-Inflammatory Activity of Arctostaphylos uva-ursi Leaf Extract against Cutibacterium acnes
IF: 6.525 -
Comparative Study of the Pharmacological Properties and Biological Effects of Polygonum aviculare L. herba Extract-Entrapped Liposomes versus Quercetin-Entrapped Liposomes on Doxorubicin-Induced Toxicity on HUVECs
IF: 6.321 -
Whole grain germinated brown rice regulates intestinal immune homeostasis and gastrointestinal hormones in type 2 diabetic patients—a randomized control trial
IF: 6.317Journal:Food & Function -
Novel insights into the role of BRD4 in fine particulate matter induced airway hyperresponsiveness
IF: 6.291
E-UNEL-H0097 | Uncoated Human IL-6(Interleukin 6) ELISA Kit | 96T*5 , 96T*15 |