Mitochondrial Membrane Potential Assay Kit (with JC-1)
中文名称：线粒体膜电位检测试剂盒(JC-1)
Price:
- 应用： 细胞凋亡-JC-1
| 检测原理 | 线粒体膜电位下降是细胞凋亡早期的标志性事件，发生在细胞核凋亡特征（染色质浓缩、DNA断裂）出现之前，一旦线粒体膜电位崩溃，细胞凋亡便不可逆转。
JC-1是一种广泛用于检测线粒体膜电位?Ψm的理想荧光探针，可以检测细胞、组织或纯化的线粒体膜电位。JC-1有单体和多聚体两种存在形式，两者发射光谱不同。在正常细胞内，线粒体膜电位较高，JC-1聚集在线粒体的基质中，形成聚合物，可产生红色荧光；凋亡早期，线粒体膜电位降低，JC-1不能聚集在线粒体基质中，此时JC-1为单体，可产生绿色荧光。
通过JC-1从红色荧光到绿色荧光的转变可检测到细胞膜电位的下降，可将JC-1荧光颜色的转变作为细胞凋亡早期的检测指标。常用红绿荧光的相对比例来衡量线粒体去极化的比例。
JC-1单体的最大激发波长为514 nm，最大发射波长为529 nm；JC-1聚合物的最大激发波长为585 nm，最大发射波长为590 nm。实际观察时，常规设置红色荧光和绿色荧光即可。 |
| 检测方法 | 荧光;流式 |
| 样本类型 | 细胞 |
| 检测时间 | 40min |
| 检测仪器 | 流式细胞仪;荧光显微镜 |
| 荧光 | JC-1 |
| Ex/Em | 514/529, 585/590 |
| Channel set | FITC, PE |
| Filter set | FITC, TRITC |
| 自备试剂 | 超纯水 |
| 保存温度 | 该产品可在2~8°C/-20°C避光条件下保存12个月，具体试剂组分的保存条件请参考说明书。 |
| 有效期 | 12个月 |
| 运输条件 | 冰袋 |
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| E-CK-A211 | Annexin V-FITC/PI Apoptosis Kit | 200Assays , 100Assays , 50Assays , 20Assays |
| E-CK-A320 | One-step TUNEL In Situ Apoptosis Kit (Green, FITC) | 100Assays , 50Assays , 20Assays |
| E-CK-A362 | Enhanced Cell Counting Kit 8 (WST-8/CCK8) | 500Tests , 100Tests , 500Tests×20 |
Q1:Is it able to measure red fluorescence only when operating JC-1 assay?
JC-1 exists in monomer and polymer form, and their emission spectra are different. In normal cells with high mitochondrial membrane potentia, JC-1 accumulates in the matrix of mitochondria as polymer, which producing red fluorescence. Since the decreasing of mitochondrial membrane potential during early stage of apoptosis, JC-1 can no longer be accumulated in the mitochondrial matrix, that becomes monomer with green fluorescence. Therefore, both red and green fluorescence need to be observed.
Q2:Is JC-1 kit able to detect tissue samples or tissue section samples?
It is not recommended to do so. Cells may suffer apoptosis or damage due to mechanical manipulation during preparation into cell suspension, in which case the results of JC-1 may be inaccurate. In addition, this kit can only detect live single-cell suspensions and cannot be used to slice samples.
Q3:Is it able to use E-CK-A301 for cells with GFP?
JC-1 needs to detect the changes of fluorescence signal in both Ex/Em = 514/529 and Ex/Em = 585/590 at the same time to determine the changes of mitochondrial membrane potential, and there is fluorescence interference with GFP, so this kit cannot be applied to cells with GFP.
