Mouse IL-17A(Interleukin 17A) ELISA Kit
中文名称:小鼠白介素17A(IL-17A)酶联免疫吸附测定试剂盒
别称:
CTLA8、CTLA-8、CTLA8cytotoxic T-lymphocyte-associated serine esterase 8、Cytotoxic T-lymphocyte-associated antigen 8、IL17、IL-17、IL17A、IL-17A、IL-17Acytotoxic T-lymphocyte-associated protein 8、IL-17CTLA-8
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 15.63-1000 pg/mL
- 灵敏度: 9.38 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-17A抗体包被于酶标板上,实验时样品(或标准品)中的小鼠IL-17A会与包被抗体结合。后依次加入生物素化的抗小鼠IL-17A抗体和辣根过氧化物酶标记的亲和素,抗小鼠IL-17A抗体与结合在包被抗体上的小鼠IL-17A结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IL-17A浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-17A的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | Colormetric |
检测范围 | 15.63-1000 pg/mL |
灵敏度 | 9.38 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的小鼠IL-17A,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
JMJD3/H3K27me3 epigenetic modification regulates Th17/Treg cell differentiation in ulcerative colitis
IF: 5.714 -
Mesenchymal Stem Cell-Secreted TGF-β1 Restores Treg/Th17 Skewing Induced by Lipopolysaccharide and Hypoxia Challenge via miR-155 Suppression
IF: 5.443Journal:Stem Cells International -
Divergent Effect of Tacalcitol (PRI-2191) on Th17 Cells in 4T1 Tumor Bearing Young and Old Ovariectomized Mice
IF: 5.402Journal:Aging and Disease -
Appetite Suppression and Interleukin 17 Receptor Signaling Activation of Colonic Mycobiota Dysbiosis Induced by High Temperature and High Humidity Conditions
IF: 5.293Journal:Frontiers in Cellular and Infection MicrobiologyPMID:34568080 -
PEA-OXA ameliorates allodynia, neuropsychiatric and adipose tissue remodeling induced by social isolation
IF: 5.251 -
Regulatory role of NKG2D+ NK cells in intestinal lamina propria by secreting double-edged Th1 cytokines in ulcerative colitis
IF: 5.168Journal:Oncotarget -
Human umbilical cord mesenchymal stem cells derived extracellular vesicles regulate acquired immune response of lupus mouse in vitro
IF: 4.996 -
CaMK4‐dependent phosphorylation of Akt/mTOR underlies Th17 excessive activation in experimental autoimmune prostatitis
IF: 4.966Journal:FASEB JOURNAL -
1,25(OH)2D3 inhibited Th17 cells differentiation via regulating the NF‐κB activity and expression of IL‐17
IF: 4.936Journal:CELL PROLIFERATION -
Bixin protects mice against bronchial asthma though modulating PI3K/Akt pathway
IF: 4.932