Mouse IL-17A(Interleukin 17A) ELISA Kit
中文名称:小鼠白介素17A(IL-17A)酶联免疫吸附测定试剂盒
别称:
CTLA8、CTLA-8、CTLA8cytotoxic T-lymphocyte-associated serine esterase 8、Cytotoxic T-lymphocyte-associated antigen 8、IL17、IL-17、IL17A、IL-17A、IL-17Acytotoxic T-lymphocyte-associated protein 8、IL-17CTLA-8
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 15.63-1000 pg/mL
- 灵敏度: 9.38 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-17A抗体包被于酶标板上,实验时样品(或标准品)中的小鼠IL-17A会与包被抗体结合。后依次加入生物素化的抗小鼠IL-17A抗体和辣根过氧化物酶标记的亲和素,抗小鼠IL-17A抗体与结合在包被抗体上的小鼠IL-17A结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IL-17A浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-17A的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | Colormetric |
检测范围 | 15.63-1000 pg/mL |
灵敏度 | 9.38 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的小鼠IL-17A,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
A Herbal Mixture from Propolis, Pomegranate, and Grape Pomace Endowed with Anti-Inflammatory Activity in an In Vivo Rheumatoid Arthritis Model
IF: 3.267Journal:MOLECULES -
Role of Interleukin-17 in Pathogenesis of Intestinal Fibrosis in Mice
IF: 3.199Journal:DIGESTIVE DISEASES AND SCIENCES -
Exosomes derived from synovial fibroblasts under hypoxia aggravate rheumatoid arthritis by regulating Treg/Th17 balance:
IF: 3.139Journal:EXPERIMENTAL BIOLOGY AND MEDICINE -
Gastrodin alleviates Tourette syndrome via Nrf‐2/HO‐1/HMGB1/NF‐кB pathway
IF: 2.965Journal:JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY -
Exposure time determines the protective effect of Trichinella spiralis on experimental colitis
IF: 2.914 -
RNAi Silencing of HIF-1α Ameliorates Lupus Development in MRL/lpr Mice
IF: 2.884 -
IL-17 exacerbates experimental autoimmune prostatitis via CXCL1/CXCL2-mediated neutrophil infiltration
IF: 2.775 -
AT1-receptor blockade: Protective effects of irbesartan in cardiomyocytes under hypoxic stress
IF: 2.766 -
Camelina sativa Oil Treatment Alleviates Castor Oil-Induced Diarrhea in ICR Mice by Regulating Intestinal Flora Composition
IF: 2.63Journal:Evidence-based Complementary and Alternative MedicinePMID:35178105 -
MiRNA-182-5p aggravates experimental ulcerative colitis via sponging Claudin-2
IF: 2.611