Mouse INS(Insulin) ELISA Kit
中文名称:小鼠胰岛素(INS)酶联免疫吸附测定试剂盒
别称:
IDDM2、ILPR、IRDN、MODY10
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 3.13-200 pg/mL
- 灵敏度: 1.88 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠INS抗体包被于酶标板上,实验时样品(或标准品)中的小鼠INS会与包被抗体结合。后依次加入生物素化的抗小鼠INS抗体和辣根过氧化物酶标记的亲和素,抗小鼠INS抗体与结合在包被抗体上的小鼠INS结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,INS浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中INS的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | 比色法 |
检测范围 | 3.13-200 pg/mL |
灵敏度 | 1.88 pg/mL |
样本体积 | 100μL |
样本类型 | 血清、血浆 |
特异性 | 可检测样本中的小鼠INS,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Effect of κ-carrageenan on glucolipid metabolism and gut microbiota in high-fat diet-fed mice
IF: 4.451Journal:Journal of Functional Foods -
Psoralea corylifolia L. Attenuates Nonalcoholic Steatohepatitis in Juvenile Mouse
IF: 4.4Journal:Frontiers in Pharmacology -
CXCL13 and CXCR5 are upregulated in PCOS mice ovaries but downregulated following metformin administration
IF: 4.369 -
Myrianthus arboreus P. Beauv improves insulin sensitivity in high fat diet-induced obese mice by reducing inflammatory pathways activation
IF: 4.36 -
DPP-4 inhibitor linagliptin ameliorates imiquimod-induced psoriasis-like skin alterations in type 2 diabetic mice by inhibiting the MAPK/NF-κB inflammatory pathway
IF: 4.36 -
Effects of triphenyl phosphate exposure during fetal development on obesity and metabolic dysfunctions in adult mice: Impaired lipid metabolism and intestinal dysbiosis
IF: 4.358 -
A decrease in cluster of differentiation 2 expression on natural killer cells is associated with polycystic ovary syndrome but not influenced by metformin in a mouse model
IF: 4.285Journal:BIOLOGY OF REPRODUCTION -
Modulation of Gut Microbiota by Fucoxanthin During Alleviation of Obesity in High-Fat Diet-Fed Mice
IF: 4.192 -
Sox9 is required in regeneration of pancreatic β cells following injury
IF: 4.145Journal:EXPERIMENTAL CELL RESEARCH -
Sulfathiazole treats type 2 diabetes by restoring metabolism through activating CYP19A1
IF: 4.117Journal:BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS