Mouse INS(Insulin) ELISA Kit
中文名称:小鼠胰岛素(INS)酶联免疫吸附测定试剂盒
别称:
IDDM2、ILPR、IRDN、MODY10
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 3.13-200 pg/mL
- 灵敏度: 1.88 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠INS抗体包被于酶标板上,实验时样品(或标准品)中的小鼠INS会与包被抗体结合。后依次加入生物素化的抗小鼠INS抗体和辣根过氧化物酶标记的亲和素,抗小鼠INS抗体与结合在包被抗体上的小鼠INS结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,INS浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中INS的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | 比色法 |
检测范围 | 3.13-200 pg/mL |
灵敏度 | 1.88 pg/mL |
样本体积 | 100μL |
样本类型 | 血清、血浆 |
特异性 | 可检测样本中的小鼠INS,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Progenies of gestational diabetes mellitus exhibit sex disparity in metabolism after respective therapies of insulin, glibenclamide, and metformin in dams during pregnancy
IF: 4.076 -
Rotenone protects against β-cell apoptosis and attenuates type 1 diabetes mellitus
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NRF2 mitigates acute alcohol-induced hepatic and pancreatic injury in mice
IF: 3.977Journal:FOOD AND CHEMICAL TOXICOLOGY -
Metabolomics study on liver of db/db mice treated with curcumin using UPLC-Q-TOF-MS
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Therapeutic potential of NaoXinTong Capsule on the developed diabetic nephropathy in db/db mice
IF: 3.743 -
Intra-Pancreatic Insulin Nourishes Cancer Cells: Do Insulin-Receptor Antagonists such as PGG and EGCG Play a Role?
IF: 3.682Journal:AMERICAN JOURNAL OF CHINESE MEDICINE -
Berberine attenuated olanzapine-induced metabolic alterations in mice: Targeting transient receptor potential vanilloid type 1 and 3 channels
IF: 3.647Journal:LIFE SCIENCES -
Cadmium exposure decreases fasting blood glucose levels and exacerbates type-2 diabetes in a mouse model
IF: 3.633 -
Modeling of antipsychotic-induced metabolic alterations in mice: An experimental approach precluding psychosis as a predisposing factor
IF: 3.585 -
Role of TRPV1/TRPV3 channels in olanzapine-induced metabolic alteration: Possible involvement in hypothalamic energy-sensing, appetite regulation, inflammation and mesolimbic pathway
IF: 3.347