MS Mouse TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit
中文名称:MS-小鼠肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒
别称:
DIF、TNF-alpha、TNFA、TNFSF2、TNF、TNFa
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 7.81-500 pg/mL
- 灵敏度: 4.69 pg/mL
系列介绍 | MS为Mini Sample的缩写。MS系列试剂盒的上样量仅为传统夹心法ELISA试剂盒的1/4,能有效解决实验样本体积不够的问题。Elabscience®自主开发的新技术,旨在帮助您以更高效的方式进行科学研究。 |
用途 | 试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中TNF-α浓度 |
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠TNF-α抗体包被于酶标板上,实验时样品(或标准品)中的小鼠TNF-α会与包被抗体结合。后依次加入生物素化的抗小鼠TNF-α抗体和辣根过氧化物酶标记的亲和素,抗小鼠TNF-α抗体与结合在包被抗体上的小鼠TNF-α结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,TNF-α浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中TNF-α的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | Colormetric |
检测范围 | 7.81-500 pg/mL |
灵敏度 | 4.69 pg/mL |
样本体积 | 25μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的小鼠TNF-α,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
ADAM10 suppresses demyelination and reduces seizure susceptibility in cuprizone-induced demyelination model
IF: 7.376 -
RRP Regulates Autophagy through the AMPK Pathway to Alleviate the Effect of Cell Senescence on Atherosclerosis
IF: 7.31Journal:Oxidative Medicine and Cellular Longevity -
Combination Therapy with Indigo and Indirubin for Ulcerative Colitis via Reinforcing Intestinal Barrier Function
IF: 7.31Journal:Oxidative Medicine and Cellular Longevity -
Near-infrared/pH dual-responsive nanocomplexes for targeted imaging and chemo/gene/photothermal tri-therapies of non-small cell lung cancer
IF: 7.242 -
Chitosan oligosaccharides attenuate loperamide-induced constipation through regulation of gut microbiota in mice
IF: 7.182 -
Bavachin protects against diet-induced hepatic steatosis and obesity in mice
IF: 7.169Journal:ACTA PHARMACOLOGICA SINICA -
Acute liver injury induced by carbon tetrachloride reversal by Gandankang aqueous extracts through nuclear factor erythroid 2-related factor 2 signaling pathway
IF: 7.129Journal:ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY -
Discovery of novel hybrids containing clioquinol−1-benzyl-1,2,3,6-tetrahydropyridine as multi-target-directed ligands (MTDLs) against Alzheimer's disease
IF: 7.088Journal:EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY -
Design and synthesis of hederagenin derivatives modulating STING/NF-κB signaling for the relief of acute liver injury in septic mice
IF: 7.088Journal:EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY -
Discovery of fusidic acid derivatives as novel STING inhibitors for treatment of sepsis
IF: 7.088
E-EL-M3063 | Mouse TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit | 96T , 48T , 96T*5 |