One-step TUNEL In Situ Apoptosis Kit (Red, Elab Fluor® 594)
中文名称：一步法TUNEL原位细胞凋亡检测试剂盒（红色，Elab Fluor® 594）
Price:
Size:
100Assays
50Assays
20Assays
- 应用： 细胞凋亡-DNA片段化
| 检测原理 | 细胞在发生凋亡时，会激活一些特异性的DNA内切酶，这些内切酶会切断核小体间的基因组DNA。使得凋亡细胞的DNA被切割成180~200bp片段，在琼脂糖凝胶上通常以180~200bp的阶梯状迁移。TdT酶（脱氧核糖核酸末端转移酶）将标记的dUTP连接到断裂DNA暴露的3'-OH末端，通过在这些末端添加荧光dUTP的方式来标记晚期凋亡细胞，从而可以通过荧光显微镜进行检测。 |
| 检测方法 | 荧光 |
| 样本类型 | 石蜡切片;冰冻切片;细胞爬片;细胞涂片 |
| 检测时间 | 3 hours |
| 检测仪器 | 荧光显微镜 |
| 荧光 | Elab Fluor®594 |
| Ex/Em | 590/617 |
| Filter set | TRITC |
| 自备试剂 | 4%多聚甲醛(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-BC-R187),ddH2O,含抗荧光淬灭剂的封片液(E-IR-R119),二甲苯,无水乙醇 |
| 保存温度 | 该产品可在-20°C避光条件下保存12个月。 |
| 有效期 | 12个月 |
| 运输条件 | 冰袋 |
Paraffin embedded rat heart was treated with DNase I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNase I treated sample (red). The cells were counterstained with DAPI (blue).This photo was taken by confocal microscope.
Paraffin embedded rat brain was treated with DNase I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNase I treated sample (red). The cells were counterstained with DAPI (blue).
Paraffin embedded mouse lung was treated with DNase I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNase I treated sample (red). The cells were counterstained with DAPI (blue).
Fluorescence microscope analysis of camptothecin-induced apoptosis of Hela cells.
Mixed samples of normal mouse spleen cells and DNase I treated mouse spleen cells were stained.
| E-CK-A211 | Annexin V-FITC/PI Apoptosis Kit | 200Assays , 100Assays , 50Assays , 20Assays |
| E-CK-A301 | Mitochondrial Membrane Potential Assay Kit (with JC-1) | 100Assays , 50Assays , 20Assays |
| E-CK-A321 | One-step TUNEL In Situ Apoptosis Kit (Green, Elab Fluor® 488) | 100Assays , 50Assays , 20Assays |
| E-CK-A362 | Enhanced Cell Counting Kit 8 (WST-8/CCK8) | 500Tests , 100Tests , 500Tests×20 |
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