AFP Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
20μL
- 宿主: Rabbit
- 反应性: Human;Mouse;Rat
- 应用性: WB;IHC
Verified Samples |
Verified Samples in WB:Hep G2,Mouse placenta,Rat placenta,Mouse Fetal liver,Rat Fetal liver,Mouse liver,Mouse kidney,Rat liver,Rat kidney Verified Samples in IHC:Mouse liver,Human liver cancer,Human ovarian cancer, MCF-7, MCF-7,Human liver cancer |
Dilution | WB:1:500-1:1000, IHC 1:200-1:500, IF 1:100-1:200 |
Clonality | Polyclonal |
Isotype | IgG |
Immunogen | Recombinant Mouse Afp protein expressed by E.coli |
Abbre | AFP |
Synonyms | Afp; AFPD; Alpha fetoglobulin; Alpha fetoprotein precursor; Alpha-1-fetoprotein; FETA; Hereditary persistence of alpha fetoprotein; HPAFP抗体 |
Swissprot | |
Calculated MW | 67 kDa |
Observed MW | 73 kDa |
Cellular Localization | Secreted |
Concentration | 1.1mg/mL |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Buffer | PBS with 0.05% Proclin300 and 50% glycerol, pH7.4. |
Purification Method | Antigen Affinity Purification |
Research Areas | Cancer; Cardiovascular; Developmental Biology |
Conjugation | Unconjugated |
Background | This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. |
Western blot with Afp Polyclonal antibody at dilution of 1:1000.lane 1:Hep G2 whole cell lysate,lane 2:Mouse placenta,lane 3:Rat placenta,lane 4:Mouse Fetal liver,lane 5:Rat Fetal liver,lane 6: Mouse liver,lane 7:Mouse kidney,lane 8:Rat liver,lane 9:Rat kidney
Immunohistochemistry of paraffin-embedded Mouse liver using AFP Polyclonal Antibody at dilution of 1:50
E-IR-R217 | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) | 18mL , 6mL , 3mL |
E-IR-R220 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) | 10mL , 3mL |
E-IR-R221 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) | 10mL , 3mL |
E-IR-R304A | Western Blot Detection Kit | 50Assays |
E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |