Annexin V-FITC/PI Apoptosis Kit
中文名称:Annexin V-FITC / PI细胞凋亡检测试剂盒
Price:
Size:
200Assays
100Assays
50Assays
20Assays
- 应用: 细胞凋亡-Annexin V
检测原理 | Annexin V是一种钙离子依赖性磷脂结合蛋白,与磷脂酰丝氨酸PS有高度亲和力,可通过细胞外侧暴露的PS与凋亡早期细胞胞膜结合,将Annexin V标记荧光染料,利用流式细胞仪检测细胞凋亡。
细胞核染料(PI,DAPI,7-AAD)可与双链DNA特异性结合,并产生强烈的荧光,正常情况下无法透过细胞膜。由于凋亡晚期或坏死细胞膜丧失完整性,细胞核染料(DAPI、PI、7-AAD)可进入细胞内对DNA进行染色,与Annexin V搭配使用,可区分处于不同凋亡时期的细胞。 |
检测方法 | 荧光;流式 |
样本类型 | 细胞 |
检测时间 | 40min |
检测仪器 | 流式细胞仪 |
荧光 | FITC |
Ex/Em | 490/530 |
Channel set | FITC |
自备试剂 | PBS(E-BC-R187), 去离子水 |
保存温度 | 该产品可在 2~8°C避光条件下保存12个月。 |
有效期 | 12个月 |
运输条件 | 冰袋 |
-
Potential Crosstalk between Liver and Extra-liver Organs in Mouse Models of Acute Liver Injury
IF: 4.858Journal:International Journal of Biological Sciences -
Demethoxycurcumin analogue DMC-BH inhibits orthotopic growth of glioma stem cells by targeting JNK/ERK signaling
IF: 4.831 -
Ginsenoside Rg3 inhibits renal cell carcinoma cell migration, invasion, colony formation, and tube formation and enhances apoptosis through promoting the DNA demethylation and histone acetylation
IF: 4.81 -
miR-146a-5p Promotes Chondrocyte Apoptosis and Inhibits Autophagy of Osteoarthritis by Targeting NUMB:
IF: 4.634Journal:Cartilage -
Koumine induces apoptosis in Cyprinus carpio liver cells by regulating JAK-STAT and p53 signaling pathways
IF: 4.622Journal:FISH & SHELLFISH IMMUNOLOGY -
Dihydrokaempferol attenuates CCl4-induced hepatic fibrosis by inhibiting PARP-1 to affect multiple downstream pathways and cytokines
IF: 4.46Journal:TOXICOLOGY AND APPLIED PHARMACOLOGY -
Semaphorin 3A attenuates the hypoxia suppression of osteogenesis in periodontal ligament stem cells
IF: 4.419 -
Natural-like Chalcones with Antitumor Activity on Human MG63 Osteosarcoma Cells
IF: 4.412 -
Activation of PPAR γ Protects Obese Mice from Acute Lung Injury by Inhibiting Endoplasmic Reticulum Stress and Promoting Mitochondrial Biogenesis
IF: 4.385 -
l-Carnitine reduces reactive oxygen species/endoplasmic reticulum stress and maintains mitochondrial function during autophagy-mediated cell apoptosis in perfluorooctanesulfonate-treated renal tubular cells
IF: 4.38
E-CK-A111 | Annexin V-FITC Reagent | 500Tests , 200Tests , 100Tests , 50Tests |
E-CK-A151 | Annexin V Binding Buffer (10×) | 50mL , 10mL |
E-CK-A212 | Annexin V-FITC/7-AAD Apoptosis Kit | 200Assays , 100Assays , 50Assays , 20Assays |
E-CK-A252 | Annexin V-FITC/DAPI Apoptosis Kit | 200Assays , 100Assays , 50Assays , 20Assays |
E-CK-A301 | Mitochondrial Membrane Potential Assay Kit (with JC-1) | 100Assays , 50Assays , 20Assays |
E-CK-A320 | One-step TUNEL In Situ Apoptosis Kit (Green, FITC) | 100Assays , 50Assays , 20Assays |