ATG5 Monoclonal Antibody
Price:
Size:
120μL
60μL
20μL
- 宿主： Mouse
- 反应性： Human;Mouse;Rat
- 应用性： WB;IHC-p
| Verified Samples |
Verified Samples in WB:Hela Verified Samples in IHC:Rat testis,Human ovarian carcinoma |
| Dilution | WB 1:500-2000;IHC 1:50-300 |
| Clonality | Monoclonal |
| Isotype | IgG |
| Immunogen | Recombinant Protein of ATG5 |
| Abbre | ATG5 |
| Synonyms | APG 5;APG 5L;APG5;APG5 autophagy 5 like;APG5 like;APG5-like;APG5L;Apoptosis specific protein;Apoptosis-specific protein;ASP;ATG 5;Atg5;ATG5 autophagy related 5 homolog;ATG5;Autophagy protein 5;Autophagy related 5;hAPG5;Homolog of S Cerevisiae autophagy 5;OTTHUMP00000040507 |
| Swissprot | |
| Observed MW | 55kDa |
| Cellular Localization | Cytoplasm. Preautophagosomal structure membrane. Colocalizes with nonmuscle actin. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme. |
| Tissue Specificity | Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Purification Method | Protein A purification |
| Research Areas | Cancer; Cell Biology; Cardiovascular; Metabolism; Signal Transduction |
| Clone No. | 5D3 |
| Conjugation | Unconjugated |
| Background | Autophagy protein 5 is a protein that in humans is encoded by the ATG5 gene. It is an E3 ubiquitin ligase which is necessary for autophagy due to its role in autophagosome elongation. It is activated by ATG7 and forms a complex with ATG12 and ATG16L1. This complex is necessary for LC3-1 conjugation to PE to form LC3-II. |
Western Blot analysis of Hela cells using ATG5 Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Rat testis tissue using ATG5 Monoclonal Antibody at dilution of 1:200.
Immunohistochemistry of paraffin-embedded Human ovarian carcinoma tissue using ATG5 Monoclonal Antibody at dilution of 1:200.
| E-AB-1001 | Goat Anti-Mouse IgG(H+L)(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1024 | Goat Anti-Mouse IgG(H+L)(Biotin conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1196 | HRP-Goat Anti-Mouse IgG(H+L) preadsorbed | 500μL , 120μL , 1mL |
| E-IR-R217 | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) | 18mL , 6mL , 3mL |
| E-IR-R220 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) | 10mL , 3mL |
| E-IR-R221 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) | 10mL , 3mL |
| E-IR-R304A | Western Blot Detection Kit | 50Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |
