BAX Monoclonal Antibody
Price:
Size:
120μL
60μL
20μL
- 宿主: Mouse
- 反应性: Human;Mouse;Rat
- 应用性: WB;IHC-p
Verified Samples |
Verified Samples in WB:Hela,C2C12,PC12 Verified Samples in IHC:Human breast carcinoma,Mouse kidney |
Dilution | WB 1:1000-2000, IHC 1:100-200 |
Clonality | Monoclonal |
Isotype | IgG |
Immunogen | Synthetic Peptide of Bax |
Abbre | BAX |
Synonyms | Apoptosis regulator BAX;BAX;Bax-protein;BAX;BAXA;Baxdelta2G9;Baxdelta2G9omega;Baxdelta2omega;Bcl-2-like protein 4;BCL2 associated X protein;BCL2 associated X protein omega;BCL2 associated X protein transcript variant delta2;Bcl2-L-4;BCL2L4;membrane isoform alpha抗体 |
Swissprot | |
Observed MW | 20kDa |
Cellular Localization | Cytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane. |
Tissue Specificity | Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines. |
Concentration | 1 mg/mL |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Buffer | PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4 |
Purification Method | Protein A purification |
Research Areas | Cancer; Cell Biology; Metabolism |
Clone No. | Clone:1D8 |
Conjugation | Unconjugated |
Background | Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. |
Western Blot analysis of 1) Hela, 2) C2C12, 3) PC-12 cells using Bax Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human breast carcinoma tissue using Bax Monoclonal Antibody at dilution of 1:200.
Immunohistochemistry of paraffin-embedded Mouse kidney tissue using Bax Monoclonal Antibody at dilution of 1:200.
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The pivotal role of miRNA-21 in myocardial metabolic flexibility in response to short- and long-term high glucose treatment: Evidence in human cardiomyocyte cell line
IF: 8.18 -
Royal jelly protects brain tissue against fluoride-induced damage by activating Bcl-2/NF-κB/caspase-3/caspase-6/Bax and Erk signaling pathways in rats
IF: 5.19Journal:ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH -
The pro-apoptotic effect of a Terpene-rich Annona cherimola leaf extract on leukemic cell lines
IF: 2.479
E-IR-R217 | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) | 18mL , 6mL , 3mL |
E-IR-R220 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) | 10mL , 3mL |
E-IR-R221 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) | 10mL , 3mL |
E-IR-R304A | Western Blot Detection Kit | 50Assays |
E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |