Cyt-C Polyclonal Antibody
Price:
Size:
100μL
50μL
- 宿主： Rabbit
- 反应性： Human;Mouse
- 应用性： WB
| Verified Samples |
Verified Samples in WB:HepG2,MCF-7,Jurkat,Molt-4,C2C12,Mouse brain |
| Dilution | WB 1:250-1:500 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant Human Cyt-C protein expressed by? E.coli |
| Abbre | Cyt-C |
| Synonyms | CYC;CYCS;cyto c;Cytochrome c;cytochrome c;somatic;HCS;THC4 |
| Swissprot | |
| Calculated MW | 12kDa |
| Observed MW | 14kDa |
| Concentration | 1mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide,50% glycerol pH 7.4 |
| Purification Method | Antigen Affinity Purification |
| Research Areas | Cancer;Cell Biology;Cardiovascular;Metabolism;Signal Transduction |
| Conjugation | Unconjugated |
| Background | Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. As a part of respiratory chain,cytochrome c plays a critical role in the process of oxidative phosphorylation and ATP producing. Besides,cytochrome c also gets implicated in apoptosis process. Upon apoptotic stimulation,cytochrome c can be released from mitochondria into cytoplasm,which is required for caspase-3 activation and the occurrence of apoptosis. |
Western blot with Cyt-C Polyclonal antibody at dilution of 1:500.lane 1:HepG2 whole cell lysate,lane 2:MCF-7 whole cell lysate,lane 3:Jurkat whole cell lysate,lane 4:Molt-4 whole cell lysate,lane 5:C2C12 whole cell lysate,lane 6:Mouse brain
| E-AB-1003 | Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1194 | HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed | 500μL , 120μL , 1mL |
| E-IR-R304A | Western Blot Detection Kit | 50Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |
