Enhanced Cell Counting Kit 8 (WST-8/CCK8)
中文名称:增强型细胞活力检测试剂盒(CCK-8)
Price:
- 应用: 细胞活力/细胞增殖/细胞毒性
检测原理 | WST-8 (2-(2-甲氧基-4-硝苯基)-3-(4-硝苯基)-5-(2,4-二磺基苯)-2H-四唑单钠盐),在电子载体存在的情况下,WST-8被线粒体内的脱氢酶还原生成水溶性的橙黄色formazan,生成的formazan的量与活细胞数量成正比,通过检测在450 nm处的吸光度,可以间接计算活细胞的量。 |
检测方法 | 比色法 |
样本类型 | 细胞 |
检测仪器 | 酶标仪 |
保存温度 | 该产品可在 2~8°C或-20°C避光条件下保存24个月。 |
有效期 | 24个月 |
运输条件 | 冰袋 |
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E-CK-A211 | Annexin V-FITC/PI Apoptosis Kit | 200Assays , 100Assays , 50Assays , 20Assays |
E-CK-A301 | Mitochondrial Membrane Potential Assay Kit (with JC-1) | 100Assays , 50Assays , 20Assays |
E-CK-A320 | One-step TUNEL In Situ Apoptosis Kit (Green, FITC) | 100Assays , 50Assays , 20Assays |
E-CK-A341 | MTT Cell Proliferation and Cytotoxicity Assay Kit | 1000Assays , 500Assays |
Q1:Will the color of drug that used to stimulate cells affect the results of CCK-8 experiment?
The effect of drug color can be eliminated by changing the medium after the drug incubation and before adding CCK-8.
Q2:What are the differences among MTS, XXT, WST-1, CCK-8 (WST-8) test kits?
MTT assay kits are widely used to detect cell proliferation and cytotoxicity, and MTS, XXT, WST-1 and WST-8 are all upgraded alternatives to MTT, which have obvious advantages compared with MTT. On the one hand, the reaction production of MTT assay is insoluable in water, a specific solvent is required in the assay, products of WST-8, XTT and MTS are water-soluble with easier operation. On the other hand, the solubility of the formazan produced of WST-8 is higher than that of XTT and MTS. What's more, WST-8 is more stable than MTT, XTT, and has a wider linear range and higher sensitivity.