GAPDH Polyclonal Antibody
Price:
Size:
500μL
300μL
120μL
60μL
30μL
- 宿主: Rabbit
- 反应性: Human;Mouse;Rat
- 应用性: WB;IHC
Verified Samples |
Verified Samples in WB:A431,HepG2,Hela,HT29,A549,Jurkat,MCF-7,Rat heart,Mouse brain,Mouse heart,Rat brain Verified Samples in IHC:Human bladder cancer,Human kidney,Mouse colon,Rat colon |
Dilution | WB 1:3000-1:6000, IHC 1:300-1:500 |
一抗 | 1:5000 |
二抗 | 1:5000 |
封闭 | 2h |
Clonality | Polyclonal |
Isotype | IgG |
Immunogen | Recombinant Zebrafish Glyceraldehyde-3-phosphate dehydrogenase protein |
Abbre | GAPDH |
Synonyms | aging associated gene 9 protein; BARS-38; cb609; G3PDH; GAPD; KNC-NDS6; OCAS; p38 component抗体 |
Swissprot | |
Calculated MW | 35 kDa |
Observed MW | 35 kDa |
Cellular Localization | Cytoplasm, Cytoskeleton, Nucleus |
Concentration | 0.66mg/mL |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Buffer | PBS with 0.05% Proclin300, 50% glycerol, pH7.3. |
Purification Method | Antigen Affinity Purification |
Research Areas | Neuroscience; Isotype/Loading Controls; Signal Transduction; Cancer; Metabolism |
Conjugation | Unconjugated |
Background | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation. |
Western Blot analysis of A431 and Hela cells using GAPDH Polyclonal Antibody at dilution of 1:5000
Western Blot analysis of HepG2, HT-29, A549, Jurkat, MCF-7, Rat heart, Mouse brain, Mouse heart and Rat brain using GAPDH Polyclonal Antibody at dilution of 1:5000
Immunohistochemistry of paraffin-embedded Human bladder cancer using GAPDH Polyclonal Antibody at dilution of 1:400
Immunohistochemistry of paraffin-embedded Human kidney using GAPDH Polyclonal Antibody at dilution of 1:400
Immunohistochemistry of paraffin-embedded Mouse colon using GAPDH Polyclonal Antibody at dilution of 1:400
Immunohistochemistry of paraffin-embedded Rat colon using GAPDH Polyclonal Antibody at dilution of 1:400
-
LINC00473-modified bone marrow mesenchymal stem cells incorporated thermosensitive PLGA hydrogel transplantation for steroid-induced osteonecrosis of femoral head: A detailed mechanistic study and validity evaluation
IF: 10.711Journal:Bioengineering & Translational Medicine -
Thyroid Hormone Regulates Glutamine Metabolism and Anaplerotic Fluxes by Inducing Mitochondrial Glutamine Aminotransferase GPT2
IF: 9.423Journal:Cell Reports -
Gut Microflora Modulates Th17/Treg Cell Differentiation in Experimental Autoimmune Prostatitis via the Short-Chain Fatty Acid Propionate
IF: 8.786 -
Anti-PADI4 antibody suppresses breast cancer by repressing the citrullinated fibronectin in the tumor microenvironment
IF: 7.419 -
Azetidin-2-one-based small molecules as dual hHDAC6/HDAC8 inhibitors: Investigation of their mechanism of action and impact of dual inhibition profile on cell viability
IF: 6.514 -
Pholiota nameko Polysaccharides Protect against Ultraviolet A-Induced Photoaging by Regulating Matrix Metalloproteinases in Human Dermal Fibroblasts
IF: 6.313 -
Mechanism of antifungal activity and therapeutic action of β-ionone on Aspergillus fumigatus keratitis via suppressing LOX1 and JNK/p38 MAPK activation
IF: 5.714 -
Dimethyl fumarate ameliorates fungal keratitis by limiting fungal growth and inhibiting pyroptosis
IF: 5.714Journal:INTERNATIONAL IMMUNOPHARMACOLOGY -
Exosomes Derived From Human Gingival Mesenchymal Stem Cells Attenuate the Inflammatory Response in Periodontal Ligament Stem Cells
IF: 5.221 -
Hypoxia and Porphyromonas gingivalis-lipopolysaccharide synergistically induce NLRP3 inflammasome activation in human gingival fibroblasts
IF: 4.932
E-IR-R217 | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) | 18mL , 6mL , 3mL |
E-IR-R220 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) | 10mL , 3mL |
E-IR-R221 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) | 10mL , 3mL |
E-IR-R304A | Western Blot Detection Kit | 50Assays |
E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |