GRP78 Polyclonal Antibody
Price:
Size:
200μL
100μL
50μL
20μL
- 宿主: Rabbit
- 反应性: Human;Mouse;Rat
- 应用性: WB
Verified Samples |
Verified Samples in WB:Hela,Jurkat,MCF-7,Mouse lung,Mouse liver,Rat lung,Rat liver,Rat small intestine |
Dilution | WB 1:500-1000 |
Clonality | Polyclonal |
Isotype | IgG |
Immunogen | Recombinant Human GRP78 protein expressed by E.coli. |
Abbre | GRP78 |
Synonyms | BiP; GRP-78; HSP70 family protein 5; Heat shock protein family A member 5; Immunoglobulin heavy chain-binding protein; HSPA5抗体 |
Swissprot | |
Calculated MW | 72kDa |
Observed MW | 78kDa |
Cellular Localization | Cytoplasm,Endoplasmicreticulumlumen,Cytoplasm,Cellsurface |
Concentration | 1.5mg/mL |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Buffer | PBS with 0.02% sodium azide, 50% glycerol,pH 7.4 |
Purification Method | Antigen Affinity Purification |
Research Areas | Cancer; Tags and Cell Markers |
Conjugation | Unconjugated |
Background | GRP78 (Glucose-regulated protein 78 kDa; also BiP and HSPA5) is a 72 kDa member of the heat shock protein 70 family of proteins. Intracellularly, GRP78 is an endoplasmic reticulum chaperone that participates in protein folding; extracellularly, it induces IL-10 production from T cells and interacts with Cripto to block TGF-beta signaling. Human GRP78 precursor is 654 amino acids (aa) in length. It contains an 18 aa signal sequence and a 636 aa mature region that shows a hydantoinase A region (aa 145245) and a C-terminal KDEL motif that is present on intracellular GRP78, but absent on secreted GRP78. There is alternative splicing in the signal sequence (aa 110), and multiple single aa substitituion. Over aa 1654, human GRP78 is more than 97% aa identical to mouse and rat GRP78. |
Western blotting with anti-GRP78 polyclonal antibody at dilution of 1:1000. Lane 1: Hela cell lysate, Lane 2: Jurkat cell lysate, lane 3: MCF-7 cell lysate, Lane 4: Mouse lung lysate, Lane 5: Mouse liver lysate, Lane 6: Rat lung lysate, Lane 7: Rat liver lysate, Lane 8: Rat small intestine lysate.
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Upregulation of AGR2vH facilitates cholangiocarcinoma cell survival under endoplasmic reticulum stress via the activation of the unfolded protein response pathway
IF: 2.928Journal:INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
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