One-step TUNEL In Situ Apoptosis Kit (Green, Elab Fluor® 488)
中文名称:一步法TUNEL原位细胞凋亡检测试剂盒(绿色,Elab Fluor® 488)
Price:
Size:
100Assays
50Assays
20Assays
- 应用: 细胞凋亡-DNA片段化
检测原理 | 细胞在发生凋亡时,会激活一些特异性的DNA内切酶,这些内切酶会切断核小体间的基因组DNA。使得凋亡细胞的DNA被切割成180~200bp片段,在琼脂糖凝胶上通常以180~200bp的阶梯状迁移。TdT酶(脱氧核糖核酸末端转移酶)将标记的dUTP连接到断裂DNA暴露的3'-OH末端,通过在这些末端添加荧光dUTP的方式来标记晚期凋亡细胞,从而可以通过荧光显微镜进行检测。 |
检测方法 | 荧光 |
样本类型 | 石蜡切片;冰冻切片;细胞爬片;细胞涂片 |
检测时间 | 3 hours |
检测仪器 | 荧光显微镜 |
荧光 | Elab Fluor®488 |
Ex/Em | 495/519 |
Channel set | FITC |
Filter set | FITC |
自备试剂 | 4%多聚甲醛(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-BC-R187),ddH2O,含抗荧光淬灭剂的封片液(E-IR-R119),二甲苯,无水乙醇 |
保存温度 | 该产品可在-20°C避光条件下保存12个月。 |
有效期 | 12个月 |
运输条件 | 冰袋 |
Paraffin embedded rat heart was treated with DNAse I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNAse I treated sample (green). The cells were counterstained with DAPI (blue).This photo was taken by confocal microscope.
Paraffin embedded rat brain was treated with DNAse I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNAse I treated sample (green). The cells were counterstained with DAPI (blue).
Paraffin embedded mouse colon was treated with DNAse I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNAse I treated sample (green). The cells were counterstained with DAPI (blue).
Fluorescence microscope analysis of camptothecin-induced apoptosis of Hela cells.
Mixed samples of normal mouse spleen cells and DNase I treated mouse spleen cells were stained.
E-CK-A211 | Annexin V-FITC/PI Apoptosis Kit | 200Assays , 100Assays , 50Assays , 20Assays |
E-CK-A301 | Mitochondrial Membrane Potential Assay Kit (with JC-1) | 100Assays , 50Assays , 20Assays |
E-CK-A320 | One-step TUNEL In Situ Apoptosis Kit (Green, FITC) | 100Assays , 50Assays , 20Assays |
E-CK-A362 | Enhanced Cell Counting Kit 8 (WST-8/CCK8) | 500Tests , 100Tests , 500Tests×20 |
Q1:If the frozen section have been fixed before freezing, do they still need to be fixed and incubated at room temperature for 30min?
If it has been fixed, it is not necessary to incubate and fix again, and subsequent experimental steps can be directly carried out.
Q2:Is the cell slides/smears for TUNEL assay able to be stored overnight after fixation?
Yes, soak in PBS and store at 4℃.