PCNA Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
20μL
- 宿主: Rabbit
- 反应性: Human; Mouse; Rat
- 应用性: WB;IHC
Verified Samples |
Verified Samples in WB:293T,HepG2 Verified Samples in IHC:Human prostate cancer,Human brain |
Dilution | WB 1:500-1:2000, IHC 1:25-1:100 |
Clonality | Polyclonal |
Isotype | IgG |
Immunogen | Fusion protein of human PCNA |
Abbre | PCNA |
Synonyms | ATLD2;cb16;Cyclin;DNA polymerase delta auxiliary protein;etID36690.10;fa28e03;fb36g03;HGCN8729;MGC8367;Mutagen-sensitive 209 protein;OTTHUMP00000030189;OTTHUMP00000030190;PCNA;Pcna/cyclin;PCNA;PCNAR;Polymerase delta accessory protein;Proliferating cell nuclear antigen;wu:fa28e03;wu:fb36g03抗体 |
Swissprot | |
Calculated MW | 29 kDa |
Observed MW | Refer to figures |
Cellular Localization | Nucleus |
Concentration | 0.7 mg/mL |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
Purification Method | Antigen affinity purification |
Research Areas | Cancer; Cell Biology; Epigenetics and Nuclear Signaling; Tags and Cell Markers |
Conjugation | Unconjugated |
Background | Proliferating Cell Nuclear Antigen,commonly known as PCNA,is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase,but not apurinic-apyrimidinic (AP) endonuclease,APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. |
Western blot analysis of 293T and HEPG2 cell lysates using PCNA Polyclonal Antibody at dilution of 1:400
Immunohistochemistry of paraffin-embedded Human prost at e cancer tissue using PCNA Polyclonal Antibody at dilution of 1:35(×200)
Immunohistochemistry of paraffin-embedded Human brain tissue using PCNA Polyclonal Antibody at dilution of 1:35(×200)
E-IR-R217 | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) | 18mL , 6mL , 3mL |
E-IR-R220 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) | 10mL , 3mL |
E-IR-R221 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) | 10mL , 3mL |
E-IR-R304A | Western Blot Detection Kit | 50Assays |
E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |