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PCNA Polyclonal Antibody

Price: ¥2365 ¥1640 ¥1010 ¥420

Size:
200μL 120μL 60μL 20μL
  • 宿主: Rabbit
  • 反应性: Human;Mouse;Rat
  • 应用性: WB;IHC
Verified Samples
Verified Samples in WB:HepG2,Hela,RAW264.7,Raji,MCF-7
Verified Samples in IHC:Human colon cancer,Mouse testis,Rat testis
Dilution
WB 1:500-1:2000 , IHC 1:50-1:200
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant Human Proliferating cell nuclear antigen protein
Abbre
PCNA
Synonyms
ATLD2; cb16; Cyclin; DNA polymerase delta auxiliary protein; etID36690.10; fa28e03; fb36g03; HGCN8729; MGC8367; Mutagen-sensitive 209 protein; OTTHUMP00000030189; OTTHUMP00000030190; PCNA; Pcna/cyclin; PCNAR; Polymerase delta accessory protein抗体
Swissprot
Calculated MW
28 kDa
Observed MW
34 lDa
Cellular Localization
Nucleus
Concentration
0.28mg/mL
Storage
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer
PBS with 0.05% Proclin300, 50% glycerol, pH7.3.
Purification Method
Antigen Affinity Purification
Research Areas
Cell Biology; Epigenetics and Nuclear Signaling; Tags and Cell markers; Cancer; Isotype/Loading Controls
Conjugation
Unconjugated
Background
This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
Western Blot analysis of HepG2, Hela, Raw264.7 Raji and MCF-7 cells using PCNA Polyclonal Antibody at dilution of 1:500
Immunohistochemistry of paraffin-embedded Human colon cancer using PCNA Polycloanl Antibody at dilution of 1:50
Immunohistochemistry of paraffin-embedded Mouse testis using PCNA Polycloanl Antibody at dilution of 1:50
Immunohistochemistry of paraffin-embedded Rat testis using PCNA Polycloanl Antibody at dilution of 1:50


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