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PCNA Polyclonal Antibody

Price: ¥2835 ¥1975 ¥875 ¥548 ¥295

Size:
500μL 300μL 120μL 60μL 30μL
  • 宿主: Rabbit
  • 反应性: Human
  • 应用性: WB
Verified Samples
Verified Samples in WB:Jurkat, A431,293T
Dilution
WB 1:3000-1:7000
一抗
1:6000
二抗
1:5000
封闭
2h
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide of Human Proliferating cell nuclear antigen
Abbre
PCNA
Synonyms
ATLD2; cb16; Cyclin; DNA polymerase delta auxiliary protein; etID36690.10; fa28e03; fb36g03; HGCN8729; MGC8367; Mutagen-sensitive 209 protein; PCNA; Pcna/cyclin; PCNA; PCNAR; Polymerase delta accessory protein; Proliferating cell nuclear antigen抗体
Swissprot
Calculated MW
28 kDa
Observed MW
35 kDa
Cellular Localization
Nucleus
Tissue Specificity
Highest expressed in oocyte
Concentration
1mg/mL
Storage
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer
PBS with 0.05% Proclin300, 50% glycerol, pH7.3.
Purification Method
Antigen Affinity Purification
Research Areas
Cancer; Cell Biology; Epigenetics and Nuclear Signaling; Tags and Cell Markers
Conjugation
Unconjugated
Background
Proliferating Cell Nuclear Antigen,commonly known as PCNA,is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase,but not apurinic-apyrimidinic (AP) endonuclease,APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells.
Western Blot analysis of Jurkat, A431 and 293T cells using PCNA Polyclonal Antibody at dilution of 1:6000.


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