PTEN Polyclonal Antibody

Verified Samples in WB:HeLa,Mouse liver,Mouse intestine,Mouse spleen,Rat liver
Verified Samples in IF: U2OS

WB 1:500-1:2000, IF 1:50-1:200

Polyclonal

IgG

Recombinant fusion protein of human PTEN (NP_000305.3).

PTEN

10q23del;BZS;CWS1;DEC;GLM2;MHAM;MMAC1;PTEN1;TEP1;PTEN;PTENbeta抗体

19kDa/47kDa/64kDa

58kDa

Secreted. May be secreted via a classical signal peptide and reenter into cells with the help of a poly-Arg motif and Cytoplasm. Nucleus. Nucleus, PML body. Monoubiquitinated form is nuclear. Nonubiquitinated form is cytoplasmic. Colocalized with PML and USP7 in PML nuclear bodies. XIAP/BIRC4 promotes its nuclear localization.

1mg/mL

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Affinity purification

Cancer; Cell Biology; Epigenetics and Nuclear Signaling; Metabolism; Signal Transduction

Unconjugated

This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.