RBFOX3 Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
- 宿主： Rabbit
- 反应性： Human;Mouse;Rat
- 应用性： WB
| Verified Samples |
Verified Samples in WB:U87,SH-SY5Y,Human brain,Mouse brain,Mouse cortex,Mouse hippocampal,Rat brain,Rat cortex,Rat hippocampal |
| Dilution | WB 1:500-1:2000 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant protein corresponding to Mouse NEUN |
| Abbre | RBFOX3 |
| Synonyms | FLJ56884;FLJ58356;Fox-1 homolog C;fox1 homolog C;Fox3;FOX3NeuN;hexaribonucleotide binding protein 3;HRNBP3;NEUN;neuronal nuclei;Rbfox3;RFOX3;RNA binding protein fox-1 homolog 3;RNA binding protein;fox 1 homolog (C. elegans) 3 |
| Swissprot | |
| Calculated MW | 46-48kDa |
| Observed MW | 46-48kDa |
| Cellular Localization | Cytoplasm, Nucleus |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH7.4 |
| Purification Method | Affinity purification |
| Research Areas | Cancer; Epigenetics and Nuclear Signaling; Neuroscience; Tags and Cell Markers |
| Conjugation | Unconjugated |
| Background | Immunoprecipitation and mass spectrometry of the two major NeuN species at 45–50 kDa identified both as the RNA binding protein Rbfox3 (a member of the Fox family of alternative splicing factors), confirming and extending the identification of the 45 kDa band as Rbfox3. Mapping of the anti-NeuN reactive epitopes in both R3hdm2 and Rbfox3 reveals a common proline- and glutamine-rich domain that lies at the N-terminus of the Rbfox3 protein. Nuclear Rbfox3 isoforms can also enhance the inclusion of cryptic exons in the Rbfox2 mRNA, resulting in nonsense-mediated decay of the message, thereby contributing to the negative regulation of Rbfox2 by Rbfox3 through a novel mechanism. |
Western Blot analysis of various samples using RBFOX3 Polyclonal Antibody at dilution of 1:1000.
| E-AB-1003 | Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1194 | HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed | 500μL , 120μL , 1mL |
| E-IR-R304A | Western Blot Detection Kit | 50Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |
