TNF alpha Monoclonal Antibody
Price:
Size:
120μL
60μL
20μL
- 宿主： Mouse
- 反应性： Human;Mouse;Rat
- 应用性： WB;IHC-p
| Verified Samples |
Verified Samples in WB:Recombinant protein Verified Samples in IHC:Human tonsil |
| Dilution | WB 1:1000-1:3000;IHC 1:50-1:200 |
| Clonality | Monoclonal |
| Isotype | IgG |
| Immunogen | Synthetic Peptide |
| Abbre | TNF α |
| Synonyms | APC1;APC1 protein;Cachectin;DIF;Differentiation inducing factor;Macrophage cytotoxic factor;Tnf;TNF superfamily;member 2;TNF;macrophage derived;TNF;monocyte derived;TNF-a;TNF-alpha;TNFA;TNFA;TNFSF2;Tumor necrosis factor alpha;Tumor necrosis factor;Tumor necrosis factor ligand superfamily member 2;Tumor Necrosis Factor;Membrane Form;Tumor necrosis factor;soluble form抗体 |
| Swissprot | |
| Observed MW | 17,25kDa |
| Cellular Localization | Secreted and Cell membrane. |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Purification Method | Protein A purification |
| Research Areas | Cancer; Cardiovascular; Metabolism; Immunology; Signal Transduction |
| Clone No. | Q34 |
| Conjugation | Unconjugated |
| Background | TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. |
Western Blot analysis of Recombinant Human TNF a Protein using TNF alpha Monoclonal Antibody at dilution of 1:2000.
Immunohistochemistry of paraffin-embedded Human tonsil tissue using TNF alpha Monoclonal Antibody at dilution of 1:50.
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Immunoreactive definition of TNF- α, HIF-1 α, Kir6.2, Kir3.1 and M2 muscarinic receptor for cardiac and pancreatic tissues in a mouse model for type 1 diabetes
IF: 5.037 -
Nephroprotective effect of apilarnil in lipopolysaccharide-induced sepsis through TLR4/NF-κB signaling pathway
IF: 5.037
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| E-AB-1196 | HRP-Goat Anti-Mouse IgG(H+L) preadsorbed | 500μL , 120μL , 1mL |
| E-IR-R217 | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) | 18mL , 6mL , 3mL |
| E-IR-R220 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) | 10mL , 3mL |
| E-IR-R221 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) | 10mL , 3mL |
| E-IR-R304A | Western Blot Detection Kit | 50Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |
