14-3-3 epsilon Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
20μL
- 宿主： Rabbit
- 反应性： Human;Mouse;Rat
- 应用性： WB;IHC
| Verified Samples |
Verified Samples in WB:Human brain malignant glioma,Human laryngocarcinoma Verified Samples in IHC:Human colon cancer,Human lung cancer |
| Dilution | WB 1:1000-1:5000;IHC 1:100-1:300 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthetic peptide of human YWHAE |
| Abbre | YWHAE |
| Synonyms | 14 3 3 E;14 3 3 epsilon;14 3 3E;14-3-3 protein epsilon;14-3-3E;1433E;Epididymis luminal protein 2;FLJ45465;FLJ53559;HEL2;KCIP 1;KCIP1;MDCR;MDS;Mitochondrial import stimulation factor L subunit;Protein kinase C inhibitor protein1;Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein;epsilon;Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein;epsilon polypeptide;Tyrosine 3/tryptophan 5 monooxygenase activation protein epsilon polypeptide;YWHAE |
| Swissprot | |
| Calculated MW | 29kDa |
| Cellular Localization | Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| Concentration | 0.3 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% sodium azide and 50% glycerol, PH7.4 |
| Purification Method | Affinity purification |
| Research Areas | Cancer; Neuroscience; Signal Transduction; Stem Cells |
| Conjugation | Unconjugated |
| Background | This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene.? |
Western Blot analysis of Human brain malignant glioma and laryngocarcinoma tissue using 14-3-3 epsilon Polyclonal Antibody at dilution of 1:2200
Immunohistochemistry of paraffin-embedded Human colon cancer using 14-3-3 epsilon Polyclonal Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded Human lung cancer using 14-3-3 epsilon Polyclonal Antibody at dilution of 1:100
| E-AB-1003 | Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1194 | HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed | 500μL , 120μL , 1mL |
| E-IR-R217 | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) | 18mL , 6mL , 3mL |
| E-IR-R220 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) | 10mL , 3mL |
| E-IR-R221 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) | 10mL , 3mL |
| E-IR-R304A | Western Blot Detection Kit | 50Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |
