AKT1 Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
20μL
- 宿主： Rabbit
- 反应性： Human;Mouse;Rat
- 应用性： IHC
| Verified Samples |
Verified Samples in IHC:Rat brain,Mouse brain,Human prostate |
| Dilution | IHC 1:50-1:100 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant Mouse RAC-alpha serine/threonine-protein kinase protein |
| Abbre | AKT1 |
| Synonyms | AKT 1;AKT;AKT1;AKT1;MGC99656;PKB;PKB-ALPHA;PRKBA;Protein Kinase B Alpha;Protein kinase B;Proto-oncogene c-Akt;RAC Alpha;RAC;RAC-alpha serine/threonine-protein kinase;RAC-PK-alpha抗体 |
| Swissprot | |
| Cellular Localization | Cytoplasm. Nucleus. Cell membrane. Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus. |
| Concentration | 2mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Purification Method | Affinity purification |
| Research Areas | Cancer; Epigenetics and Nuclear Signaling; Metabolism; Signal transduction |
| Conjugation | Unconjugated |
| Background | The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. |
Immunohistochemistry of paraffin-embedded Rat brain using AKT1 Polyclonal Antibody at dilution of 1:50
Immunohistochemistry of paraffin-embedded Mouse brain using AKT1 Polyclonal Antibody at dilution of 1:50
Immunohistochemistry analysis of paraffin-embedded Human Prostate using AKT1 Polyclonal Antibody(Elabscience Product Detected by Lifespan).
| E-AB-1003 | Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1194 | HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed | 500μL , 120μL , 1mL |
| E-IR-R217 | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) | 18mL , 6mL , 3mL |
| E-IR-R220 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) | 10mL , 3mL |
| E-IR-R221 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) | 10mL , 3mL |
| E-IR-R304A | Western Blot Detection Kit | 50Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |
