Annexin V-APC/7-AAD Apoptosis Kit
中文名称:Annexin V-APC / 7-AAD细胞凋亡检测试剂盒(即用型Buffer)
Price:
Size:
200Assays
100Assays
50Assays
20Assays
- 应用: 细胞凋亡-Annexin V
检测原理 | Annexin V是一种钙离子依赖性磷脂结合蛋白,与磷脂酰丝氨酸PS有高度亲和力,可通过细胞外侧暴露的PS与凋亡早期细胞胞膜结合,将Annexin V标记荧光染料,利用流式细胞仪检测细胞凋亡。
细胞核染料(PI,DAPI,7-AAD)可与双链DNA特异性结合,并产生强烈的荧光,正常情况下无法透过细胞膜。由于凋亡晚期或坏死细胞膜丧失完整性,细胞核染料(DAPI、PI、7-AAD)可进入细胞内对DNA进行染色,与Annexin V搭配使用,可区分处于不同凋亡时期的细胞。 |
检测方法 | 荧光法;流式 |
样本类型 | 细胞 |
检测时间 | 40min |
检测仪器 | 流式细胞仪 |
荧光 | APC |
Ex/Em | 650/660 |
Channel set | APC |
自备试剂 | PBS(E-BC-R187), 去离子水 |
保存温度 | 该产品可在 2~8°C避光条件下保存6个月。 |
有效期 | 6个月 |
运输条件 | 冰袋 |
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E-CK-A117 | Annexin V-APC Reagent | 500Tests , 200Tests , 100Tests , 50Tests |
E-CK-A151 | Annexin V Binding Buffer (10×) | 50mL , 10mL |
E-CK-A217 | Annexin V-APC/PI Apoptosis Kit | 200Assays , 100Assays , 50Assays , 20Assays |
E-CK-A258 | Annexin V-APC/DAPI Apoptosis Kit | 200Assays , 100Assays , 50Assays , 20Assays |
E-CK-A301 | Mitochondrial Membrane Potential Assay Kit (with JC-1) | 100Assays , 50Assays , 20Assays |
E-CK-A320 | One-step TUNEL In Situ Apoptosis Kit (Green, FITC) | 100Assays , 50Assays , 20Assays |