CD274 Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
20μL
- 宿主： Rabbit
- 反应性： Human
- 应用性： WB;IHC
| Verified Samples |
Verified Samples in WB:HepG2 Verified Samples in IHC:Human brain |
| Dilution | WB 1:500-1:2000;IHC 1:50-1:100 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthetic peptide of human CD274 |
| Abbre | CD274 |
| Synonyms | B7 H;B7 H1;B7 homolog 1;B7-H1;B7H;B7H1;CD 274;CD274;CD274 antigen;CD274 molecule;MGC142294;MGC142296;OTTHUMP00000021029;PD L1;PD-L1;PD1L1;PDCD1 ligand 1;PDCD1L1;PDCD1LG1;PDL 1;PDL1;Programmed cell death 1 ligand 1;Programmed death ligand 1;RGD1566211抗体 |
| Swissprot | |
| Calculated MW | 33 kDa |
| Observed MW | Refer to figures |
| Cellular Localization | Cell membrane and Endomembrane system. |
| Concentration | 0.72 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
| Purification Method | Antigen affinity purification |
| Research Areas | Cancer; Immunology |
| Conjugation | Unconjugated |
| Background | Programmed cell death ligand 1(CD274,or B7-H1,PD-L1),is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors,the B7s are key regulators of the adaptive immune response. CD274 is suggested a negative regulator of T and B cell,and play important role in mediating tolerance of lymphocytes to self-antigens. It also involved in the costimulatory signal,essential for T-cell proliferation and production of IL10 and IFNG,in an IL2-dependent and a PDCD1-independent manner. PD-L1 is a 290 aa transmembrane protein with a calculated molecular weight of 33 kDa,the apparent molecular weight has been reported as 45-70 kDa,suggesting probable glycosylation . |
Western blot analysis of HepG2 cell lysate using CD274 Polyclonal Antibody at dilution of 1:700
Immunohistochemistry of paraffin-embedded Human brain tissue using CD274 Polyclonal Antibody at dilution of 1:30(×200)
| E-AB-1003 | Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1194 | HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed | 500μL , 120μL , 1mL |
| E-IR-R217 | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) | 18mL , 6mL , 3mL |
| E-IR-R220 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) | 10mL , 3mL |
| E-IR-R221 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) | 10mL , 3mL |
| E-IR-R304A | Western Blot Detection Kit | 50Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |
