CUL4A Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
20μL
- 宿主： Rabbit
- 反应性： Human; Mouse
- 应用性： WB;IHC
| Verified Samples |
Verified Samples in WB:231,HepG2 Verified Samples in IHC:Human esophagus cancer,Human thyroid cancer |
| Dilution | WB 1:500-1:2000;IHC 1:50-1:300 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthetic peptide of human CUL4A |
| Abbre | CUL4A |
| Synonyms | 2810470J21Rik;AW495282;CUL 4A;CUL-4A;Cul4a;Cul4a protein;CUL4A;Cullin-4A;MGC36573;MGC64071抗体 |
| Swissprot | |
| Calculated MW | 88 kDa |
| Observed MW | Refer to figures |
| Cellular Localization | Nucleus,nucleoplasm,Other locations:Cul4-RING E3 ubiquitin ligase complex,Cul4A-RING E3 ubiquitin ligase complex |
| Concentration | 1.62 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
| Purification Method | Antigen affinity purification |
| Research Areas | Cancer; Cell Biology |
| Conjugation | Unconjugated |
| Background | Cullin proteins assemble a large number of RING E3 ubiquitin ligases,participating in the proteolysis through the ubiquitin-proteasome pathway. Two cullin 4 (CUL4) proteins,CUL4A (87 kDa) and CUL4B(104 kDa),have been identified. The two CUL4 sequences are 83% identical. They target certain proteins for degradation by binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex with DDB. They form two individual E3 ligases,DDB1-CUL4ADDB2 and DDB1-CUL4BDDB2 in this process. CUL4A appeared in both the nucleus and the cytosol,suggesting a more complex mechanism for entering the nucleus. CUL4B is localized in the nucleus and facilitates the transfer of DDB1 into the nucleus independently of DDB2. |
Western blot analysis of 231 and HepG2 cell lysates using CUL4A Polyclonal Antibody at dilution of 1:600
Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CUL4A Polyclonal Antibody at dilution of 1:55(×200)
Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CUL4A Polyclonal Antibody at dilution of 1:55(×200)
| E-AB-1003 | Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1194 | HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed | 500μL , 120μL , 1mL |
| E-IR-R217 | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) | 18mL , 6mL , 3mL |
| E-IR-R220 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) | 10mL , 3mL |
| E-IR-R221 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) | 10mL , 3mL |
| E-IR-R304A | Western Blot Detection Kit | 50Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |
